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引用本文:周立斌,张 伟,王安利,马细兰,张海发,刘付永忠.饲料中锌对花鲈(Lateolabrax japonicus)幼鱼生长、免疫和组织积累的影响.海洋与湖沼,2009,40(1):42-47.
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饲料中锌对花鲈(Lateolabrax japonicus)幼鱼生长、免疫和组织积累的影响
周立斌1, 张 伟2, 王安利2, 马细兰3, 张海发4, 刘付永忠4
1.华南师范大学生命科学学院 广东省高等学校生态与环境科学重点实验室,惠州学院生命科学系;2.华南师范大学生命科学学院 广东省高等学校生态与环境科学重点实验室;3.惠州学院生命科学系;4.广东省大亚湾水产实验中心
摘要:
采用单因子实验设计方法, 进行饲料中添加锌对花鲈幼鱼生长(增重率、存活率、特定生长率和饲料效率)、免疫反应(血清溶菌酶活性和总补体活性)和组织中锌积累量影响的研究。通过在基础饲料中添加ZnSO4 使饲料中锌含量分别达到83.3、95.2、101.6、120.3、159.9mg/kg, 对花鲈幼鱼进行为期8 周的生长实验, 每个水平3 个重复, 每个养殖单元放养初始体重为(10.0±0.58)g 左右的花鲈幼鱼20 尾。饲养实验在海水网箱(1.5×1.0×1.0m3)中进行, 实验期间水温为26.5—32.5℃, 盐度为28—29, 溶解氧含量在6mg/L 左右。结果表明, 各饲料组成活率(95.00%—100.00%)无显著性差异(P>0.05)。随着饲料中锌含量从83.3mg/kg 增加到101.6mg/kg, 花鲈幼鱼的增重率和特定生长率显著升高(P<0.05), 而饲料中锌含量达到101.6mg/kg 之后, 其增重率和特定生长率变化不显著(P>0.05);以增重率和特定生长率为指标, 花鲈幼鱼最佳生长性能的饲料锌含量为103.4mg/kg 左右。各饲料组肌肉中锌积累量无显著性差异; 随着饲料中锌含量从83.3mg/kg 增加到101.6mg/kg, 花鲈幼鱼肝脏中锌积累量显著增加(P<0.05), 而饲料中锌含量达到101.6mg/kg 之后, 其肝脏中锌积累量变化不显著(P>0.05); 花鲈幼鱼获得肝脏中最大锌积累量时饲料中锌最低含量为105.1mg/kg。随着饲料中锌含量从83.3mg/kg 增加到101.6mg/kg, 花鲈幼鱼血清溶菌酶和总补体活性显著升高(P<0.05), 饲料中锌含量达到101.6mg/kg 之后, 血清溶菌酶和总补体活性变化不显著(P>0.05)。综上所述, 花鲈幼鱼生长和免疫的锌适宜添加量为105.1mg/kg 左右。
关键词:  花鲈幼鱼, 锌, 生长, 免疫, 组织积累
DOI:10.11693/hyhz200901007007
分类号:
基金项目:国家科技支撑计划课题, 2007BAD29B04 号; 广东省科技计划重大专项, 2007A03260004 号; 广东省科技计划项目,2007B020708013 号; 广东省重大科技兴海(兴渔)项目, A200501G01 号
附件
EFFECTS OF DIETARY ZINC ON GROWTH, IMMUNE RESPONSE AND TISSUE CONCENTRATION OF JUVENILE JAPANESE SEABASS LATEOLABRAX JAPONICUS
ZHOU Li-Bin,ZHANG Wei,WANG An-Li,MA Xi-Lan,ZHANG Hai-Fa,LIUFU Yong-Zhong
1.College of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education;2.Department of Life Science, Huizhou University;3.Guangdong Daya Bay Fisheries Development Center
Abstract:
An experiment was conducted to study the effects of dietary zinc on growth (weight gain, feed efficiency ratio, specific growth rate and survival rate), immune response (lysozyme activity and total complement activity) and body distribution of zinc concentration of juvenile Japanese seabass Lateolabrax japonicus. Five iso-nitrogenous and iso- energetic diets were formulated and contained 83.3, 95.2, 101.6, 120.3, 159.9mg zinc per kg diet, supplied as ZnSO4·H2O, and fed in 15 floating netcages (1.5×1.0×1.0m3) with 20 fish [initial weight: (10.0±0.58)g]; fish were fed twice daily (09:30 and 16:30) for 8 weeks, the water temperature fluctuated from 26.5℃ to 32.5℃, salinity from 28 to 29 and dissolved oxygen was approximately 6mg/L during the experimental period. Results indicate that no significant differences in survival rate (95.00%—100.00%) were found among dietary treatments (P>0.05). The weight gain rate and specific growth rate increased significantly with increasing dietary zinc from 83.3 to 101.6mg/kg (P<0.05), but no significant differences were observed among the fish fed the diets with equal to or higher than 101.6mg/kg of zinc (P>0.05), and the optimal dietary zinc content for maximum growth was about 103.4mg/kg. No significant differences in zinc concentration of muscle were found among dietary treatments (P>0.05), zinc concentration in liver increased significantly with increasing dietary zinc from 83.3 to 101.6mg/kg (P<0.05); no significant differences were observed among the fish fed the diets with equal to or higher than 101.6mg/kg of zinc (P>0.05), juvenile Japanese seabass maintained greatest zinc concentration in liver and lowest zinc demand content when fed with 105.1mg/kg of dietary zinc. Serum lysozyme activity and total complement activity of juvenile Japanese seabass increased significantly with increasing dietary zinc from 83.3 to 101.6mg/kg (P<0.05), but no significant differences were observed among the fish fed with the diets with equal to or higher than 101.6mg/kg of zinc (P>0.05). Based on the information above, the optimal dietary zinc content for optimum growth performance and immune response in juvenile Japanese seabass was about 105.1mg/kg of dietary zinc.
Key words:  Japanese seabass Lateolabrax japonicus, Zinc, Growth, Immune response, Tissue concentration
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