| 摘要: |
| 用60Co-γ射线辐照处理野生型条斑紫菜的壳孢子萌发体以获得色素变异细胞, 随后用酶解法获得单离的变异细胞进行个体再生培养, 从中分离出不同的条斑紫菜色素突变体。结果表明, 叶状体经剂量分别为100、300、500、700 和900Gy 的γ射线辐照后再培养1 周, 均出现了多种色素变异细胞, 随后, 它们逐渐分裂成为变异细胞块。在0—500Gy 辐照剂量范围内, 色素变异细胞块的百分率随着辐照剂量的增加而增加, 但增至700Gy 时, 变异细胞块的百分率反而下降, 说明500Gy 是最合适的剂量。从单离变异细胞的再生叶状体中分离出桔红、紫红、桔黄和墨绿等颜色的单色变异体,并获得了它们的丝状体纯系。各变异品系的F1 叶状体均为单色, 其颜色与各自的母本叶状体相同,表明它们是稳定突变体。各色素突变品系的叶状体活体吸收光谱不仅与野生型品系有明显的差异,而且各突变品系之间也存在较大的差异。
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| 关键词: 条斑紫菜, 叶状体, 60Co-γ射线, 色素突变体, 活体吸收光谱 |
| DOI：10.11693/hyhz200901009009 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863 计划)项目资助, 2006AA10A413 号; 国家自然科学基金资助项目, 30571443 号; 上海市优秀学科带头人项目, 07XD14028 号; 上海市自然科学基金资助项目, 05RZ14110 号; 上海市教委重点学科建设项目, J50701 号 |
附件 |
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| INDUCTION AND ISOLATION OF PIGMENTATION MUTANTS OF PORPHYRA YEZOENSIS UEDA (BANGIALES, RHODOPHYTA) BY 60Co-γ RAY IRRADIATION |
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YAN Xing-Hong, ZHANG Shu-Juan, HUANG Lin-Bin
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College of Fisheries and Life Science, Shanghai Ocean University
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| Abstract: |
| To obtain pigmentation mutants of Porphyra yezoensis Ueda, single somatic cells were isolated enzymatically from the 60Co-γ-ray-irradiated conchosporlings of the wild-type strain and were cultured to regenerate into whole blades. After irradiated at 100, 300, 500, 700, and 900Gy and cultured for 1 week, the color-mutated cells in different colors appeared in the irradiated blades. These cells formed cell-clusters in the next three weeks of culture. As irradiation dosage increased from 0 to 500Gy, the frequency of the color-mutated cell-clusters increased; however, beyond 700Gy, the frequency decreased. Therefore, 500Gy is the optimal dosage for inducing mutations. The frequency of the color-mutated cell-clusters varied in different parts of blade, increased from the base to the tip of the blade. Many mutant blades in different colors appeared in the regenerated blades from the single cells. Five pigmentation mutants (red orange, purplish red, yellow orange, olive, and green color) were isolated and their free-living conchocelis were established by sexual reproduction. The F1 gametophytic blades of each mutant strains showed only one color that the same to its mother blade, indicating they have become relatively stable pigmentation mutants. Each mutant strain featured own characteristics in absorption spectra of in vivo blades, different from each other and from wild type’s.
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| Key words: Porphyra yezoensis, Gametophytic blade, 60Co- γ ray, Pigmentation mutant, In vivo absorption spectra |
