| 摘要: |
| 从角叉菜中提取λ-卡拉胶, 利用密闭微波法进行降解, 得到5 种不同分子量的产品: 650、240、140、15、9.3 kDa。红外、紫外和化学分析结果表明,这些卡拉胶样品具有相似的组成和结构, 这表明微波法不会改变多糖的组成和结构。体外实验结果表明, 角叉菜λ-卡拉胶及其降解产物对超氧阴离子(O2?)、有机自由基DPPH、H2O2诱导的小鼠红细胞氧化溶血以及小鼠肝匀浆脂质过氧化作用均有不同程度的抑制作用, 其中对小
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| 关键词: 角叉菜, λ-卡拉胶, 微波降解, 分子量, 抗氧化 |
| DOI：10.11693/hyhz200905004004 |
| 分类号: |
| 基金项目:山东省中青年科学家科研奖励基金项目, 2008BS06012 号和中国科学院海洋生物资源可持续利用重点实验室开放基金资助项目, LMB071005 |
附件 |
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| ANTIOXIDANT ACTIVITY OF Λ-CARRAGEENANS OF DIFFERENT MOLECULAR WEIGHTS FROM CHONDRUS OCELLATUS |
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ZHOU Ge-Fei1, XING Rong-Lian1, SUN Li-Qin1, TENG Li1, ZHANG Quan-Bin2, XU Zu-Hong2
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1.Ocean School of Yantai University;2.Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| The antioxidant activity of λ-carrageenans from chondrus ocelots in different molecular weights was studied. λ-carrageenan was extracted from Chandra’s ocelots and degraded in microwave from which products in different molecular weight: 650, 240, 140, 15, 9.3 kDa, were yielded. Analyses in IR, UV and chemistry demonstrated that these products are similar in chemical components and structure, showing that microwave degradation would not change the chemical components and structure of polysaccharides. In addition, in vitro scavenging activities of these λ-carrageenans showed protections of different degrees by these polysaccharides with antioxidant activities against superoxide radical, DPPH, and H2O2-induced hemolysis of rat erythrocytes, especially lipid peroxide of rat liver homogenate.
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| Key words: Chandra’s ocelots, λ-carrageenans, Microwave degradation, Molecular weights antioxidant |
