| 摘要: |
| 采用24 条随机引物对曼氏无针乌贼1 个野生群体和5 代养殖群体共 120 个个体(每个群体20 个个体)进行了RAPD 群体遗传多样性分析, 共扩增出119 个位点, 片段大小为400—2800bp, 平均每条引物的扩增带数为4.96 条。各群体的多态位点数19—24 个不等, 多态位点比例为13.33%—20.16%, Shannon 指数为0.0768—0.1018。6 个群体的平均杂合度为0.0825—0.1231, 期望杂合度为0.1325—0.1524, 平均杂合子偏离指数均为负值, 表明存在
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| 关键词: 曼氏无针乌贼, 群体, RAPD, 遗传分化 |
| DOI：10.11693/hyhz200905011011 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 40646030 号、40776076 号; 教育部长江学者和创新团队发展计划, IRT0734 号; 教育部重点科研项目, 207045 号; 浙江省重大科技专项资助, 2006C13040 号、2007C1206 号。 |
附件 |
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| GENETIC DIVERSITY OF SEPIELLA MAINDRONI IN CULTURED AND NATURAL POPULATIONS |
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SONG Wei-Wei, WANG Chun-Lin
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Faculty of Life Science and Biotechnology, Ningbo University,Key Laboratory of Applied Marine Biotechnology, Ministry of Education
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| Abstract: |
| Genetic diversity of six Sepiella maindroni stocks (20 individuals from each stock) was investigated by using 24 random RAPD primers. A total of 119 RAPD bands ranging from 400bp to 2800bp were recorded with an average of 4.96 bands gained per primer. The mean percentages of polymorphic loci of S. maindroni stocks range from 19 to 24, the proportion of polymorphic loci is 13.33% to 20.16% and the Shannon index range from 0.0768 to 0.1018. The mean value of D (heterozygote deficiency of excess) is negative, indicating the overall deficit in heterozygote for the six stocks. The genetic differentiation index FST values range from 0.0188 to 0.2436, and the most significantly diversity is between natural stock and the fifth generations of cultured stock. The results of genetic differentiation and significance analysis show that the most difference of diversity among the six stocks were from the diversity between the natural stock and cultured stocks. AMOVA results indicate that the genetic variation among stocks (29.34%) is lower than within (70.66%). If taking the five cultured stocks as a whole stock, the genetic variation among stocks (19.38%) is lower than within (80.62%). Therefore, the genetic difference between natural stock and cultured stocks has been obvious, and the diversity within the generations of cultured stocks tended to decrease especially in the fifth generation.
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| Key words: Sepiella maindroni, Population, RAPD, Genetic differentiation |
