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引用本文:王中铎,郭昱嵩,陈荣玲,何晓莹,刘楚吾,刘 筠.南海常见硬骨鱼类COⅠ条码序列.海洋与湖沼,2009,40(5):608-614.
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南海常见硬骨鱼类COⅠ条码序列
王中铎1, 郭昱嵩2, 陈荣玲2, 何晓莹2, 刘楚吾1, 刘 筠3
1.广东海洋大学水产学院,湖南师范大学生命科学院;2.广东海洋大学水产学院;3.湖南师范大学生命科学院
摘要:
运用一对特异性引物扩增并测定了南海硬骨鱼类40 个物种89 个样本的线粒体DNA 的细胞色素c氧化酶Ⅰ(COⅠ)约660bp 的部分序列, 即COⅠ条码序列。综合比较了《中国鱼类系统检索》、《海洋鱼类志》、FishBase 鱼类形态学分类库、ITIS 综合分类学信息系统和生物条码系统(The Barcode of Life Data System, BOLD)的相应形态学与DNA 序列资料, 在揭示完善我国鱼类检索系统必要性基础上, 探讨了COⅠ条码序列在硬骨鱼类辅助物种鉴别和适用性, 并对
关键词:  DNA 条码, COⅠ, 物种鉴别, 硬骨鱼类
DOI:10.11693/hyhz200905014014
分类号:
基金项目:“十一五”国家科技支撑计划项目, 2007BAD29B03 号; 广东省自然科学基金资助项目, 8452408801001272 号。
附件
COⅠBARCODING SEQUENCES OF TELEOSTS IN THE SOUTH CHINA SEA
WANG Zhong-Duo1, GUO Yu-Song2, CHEN Rong-Ling2, HE Xiao-Ying2, LIU Chu-Wu1, LIU Yun3
1.Fisheries College, Guangdong Ocean University, College of Life Science, Hunan Normal University;2.Fisheries College, Guangdong Ocean University;3.College of Life Science, Hunan Normal University
Abstract:
Forty species of teleosts in the South China Sea were sequenced (barcoded) in a 660bp region of mitochondrial cytochrome oxidase subunit I gene (COⅠ). Most of the species were represented by multiple specimens, and 89 sequences were generated. The average intra-species, -genus, -family, and -order of Kimura two parameter (K2P) distances are 0.0084, 0.1567, 0.1839 and 0.2319, respectively. All the species could be differentiated in COⅠ sequence, despite of single individuals of any two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signals are apparent in the data. In the neighbour-joining tree for all 89 sequences, species of a genera clustered invariably in three major clusters: snappers, groupers, and the other fishes. The former two clades revealed after bootstrapping generally corresponded well with expectations. It is concluded that COⅠ sequencing, or ‘barcoding’, can be used to identify fish species.
Key words:  DNA barcodes, COⅠ, Species identification, Teleosts
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