| 引用本文: | 金春华,李明云,刘伟成,张春丹,陈 炯,史雨红,朱爱意.镉胁迫对大弹涂鱼(Boleophthalmus pectinirostris)血细胞遗传损伤的研究.海洋与湖沼,2010,41(1):80-84. |
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| 摘要: |
| 以大弹涂鱼为研究对象, 应用单细胞凝胶电泳技术并结合外周血基因组DNA 琼脂糖凝胶电泳, 研究了镉胁迫对大弹涂鱼外周血细胞的遗传损伤。结果表明：(1) 镉对大弹涂鱼外周血细胞是遗传毒性而非细胞毒性, 并且产生的遗传损伤存在显著(P<0.01 或P<0.05)的剂量效应, 染毒1h 的大弹涂鱼的全长和尾长与浓度的相关方程分别是y = 29.592x－10.576 (r2 = 0.9786), y = 28.417x－14.859(r2 = 0.9857), 染毒2h 的大弹涂鱼的全长和尾长与浓度的相关方程分别是y = 32.044x－5.0235 (r2 =0.941), y = 29.911x－9.539 (r2 = 0.9635)。(2) DNA 的损伤程度和污染胁迫之间存在着时间效应。以上结果表明, DNA 损伤可作为大弹涂鱼受镉胁迫时的生物指标。
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| 关键词: 镉, 单细胞凝胶电泳(彗星试验), DNA 凝胶电泳, 大弹涂鱼 |
| DOI：10.11693/hyhz201001011011 |
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| 基金项目:长江学者和创新团队发展计划, IRT0734 号; 浙江省重大国际合作项目, 2006C14017 号。 |
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| GENTENIC DAMAGES IN PERIPHERAL BLOOD OF BOLEOPHTHALMUS PECTINIROSTRIS EXPOSED TO CADMIUM |
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JIN Chun-Hua1, LI Ming-Yun1, LIU Wei-Cheng1,2, ZHANG Chun-Dan1, CHEN Jiong1, SHI Yu-Hong1, ZHU Ai-Yi3
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1.Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and Biotechnology;2.Zhejiang Mariculture Research Institute;3.College of marine science,Zhejiang Ocean University
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| Abstract: |
| Cadmium stress on genetic damage of peripheral blood cells of marine fish Boleophthalmus pectinirostris was studied using single cell gel electrophoresis (SCGE) and DNA gel electrophoresis. The SCGE results shows that the cadmium toxicity in peripheral blood cells was not cytotoxicity but genotoxicity. Significant (P<0.05) dose-dependent correlation was found between DNA migrancy distance and cadmium concentration, and so did the time-dependent one. On the other hand. DNA repair occurred in late period with 0.1 LD50 in vitro cadmium exposure due probably to the self-immunity respond. These results suggest that the DNA damage can used as the biology markers of Cadmium stress on the B. pectinirostri. These characters made a possibility of field work, but the factor of age must be thought in field work.
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| Key words: Cadmium, Single cell gel electrophoresis (comet assay), DNA gel electrophoresis, Boleophthalmus pectiniros |
