| 摘要: |
| 对500 个成体青蛤分别用鳗弧菌和生理盐水注射, 在感染后3h、6h、12h、24h 和36h 分别取不同处理组的肝胰脏、鳃和闭壳肌组织, 测定上述样品的碱性磷酸酶(ALP)及酸性磷酸酶(ACP)活性, 分析鳗弧菌对青蛤体内免疫相关酶活性的影响。结果表明, 鳗弧菌感染组的ALP 与ACP 活性从高到低依次为肝胰脏> 鳃> 闭壳肌, 其中青蛤肝胰脏和鳃组织中ALP 和ACP 活性均有显著升高的趋势, 并且在12h 时达到最高, 与对照组差异显著(P<0.05), 随后呈现下降趋势。而青蛤闭壳肌组织中侵染组ALP 和ACP 活性与对照组之间没有显著差异(P>0.05)。鳗弧菌对青蛤的磷酸酶活性影响较大, 对其免疫防御系统有明显的刺激作用。
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| 关键词: 青蛤, 鳗弧菌, 碱性磷酸酶(ALP), 酸性磷酸酶(ACP) |
| DOI：10.11693/hyhz201002014014 |
| 分类号: |
| 基金项目:天津市科委应用基础与前沿技术重点项目资助, 09JCZDJC19300 号; 天津市科委应用基础研究面上项目资助,06YFJMJC11800 号。 |
附件 |
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| EFFECT OF VIBRIO ANGUILLARUM ON ACTIVITY OF PHOSPHATASE IN CYCLINA SINENSIS |
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SONG Xin, ZHANG Li-Yan, GAO Wei-Wei, PAN Bao-Ping
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College of Chemistry and Life Science, Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University
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| Abstract: |
| In this study, Vibrio anguillarum and normal saline were injected separately into 500 adult clams (Cyclina sinensis). At 3h, 6h, 12h, 24h, and 36h after injection, hepatopancreas, gills, and adductor muscle tissues of the samples in different treatment groups were collected, and alkaline phosphatase (ALP) and acid phosphatase (ACP) activities were measured in order to reveal the relationship between V. anguillarum injection and immune-related response. The results show that after the injection, ALP and ACP activities in the clam tissue were, in a descending order, hepatopancreas > gill > adductor muscle tissue. After the injection, the activities in hepatopancreas and gill tissues increased significantly, and reached its highest level at 12h and then decreased. In the adductor muscle tissues, the activities showed no significant difference compared with the control group. Our research shows that V. anguillarum has noticeable impact on phosphatase of C. sinensis and significant stimulating effect to the clam’s immune system.
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| Key words: Cyclina sinensis, Vibrio anguillarum, Alkaline phosphatase (ALP), Acid phosphatase (ACP) |
