引用本文:	李明云,吴玉珍,冀德伟,吴海庆,陈炯,史雨红,罗海忠,柳敏海,傅荣兵.低温选择大黄鱼(Pseudosciaena crocea)的肝脏蛋白质组双向电泳分析.海洋与湖沼,2010,41(3):348-351.

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*低温选择大黄鱼(Pseudosciaena crocea)的肝脏蛋白质组双向电泳分析*
李明云¹, 吴玉珍¹, 冀德伟¹, 吴海庆¹, 陈炯¹, 史雨红¹, 罗海忠², 柳敏海², 傅荣兵²
1.应用海洋生物技术教育部重点实验室宁波大学;2.浙江省舟山市水产研究所

摘要:

采用双向电泳技术, 对低温选择组与对照组大黄鱼进行肝脏蛋白质组双向电泳分析。结果表明, 低温选择组肝脏蛋白点1673±47 个, 对照组1651±43 个, 共有19 个蛋白点经低温选择后, 表达量显著变化。从中挑选3 个差异蛋白点进行肽指纹图谱(MALDI-TOF-MS)或串联质谱(LC-MS-MS)分析, 然后MS-BLAST 数据库搜寻。低温选择组大黄鱼肝脏MHC 和CFL2 蛋白表达显著上调, 而2-Cys Prxs 蛋白表达显著下调。说明低温选育组大黄鱼机体抗细胞凋亡能力明显增强, 预示低温选择后留下的大黄鱼在低温环境中生存能力更强。

关键词: 大黄鱼, 低温选育, 肝脏, 双向电泳

DOI：10.11693/hyhz201003008008

分类号:

基金项目:国家高技术研究发展计划(863)项目, 2006AA10A405 号; 浙江省重大项目, 2006C12005 号; 宁波市重大项目, 2008C10022号; 长江学者和创新团队发展计划项目, IRT0734 号。

附件

2-DE ANALYSIS OF LIVER OF PSEUDOSCIAENA CROCEA AFTER LOW TEMPERATURE BREEDING

LI Ming-Yun¹, WU Yu-Zhen¹, JI De-Wei¹, WU Hai-Qing¹, CHEN Jiong¹, SHI Yu-Hong¹, LUO Hai-Zhong², LIU Min-Hai², FU Rong-Bing²

1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;2.Fisheries Institute of Zhoushan of Zhejiang Province

Abstract:

We used 2-DE to compare the liver proteomes of Pseudosciaena crocea with or without low-temperature breeding, and found similar number of proteins expressed in the low-temperature breeding group (1673±47) and the control group (1651±43). The expressions of 19 proteins were significantly altered after low temperature breeding. Three proteins (MHC, CFL2 and 2-Cys Prxs) were identified by MALDI-TOF-MS/LC-MS-MS and MS-BLAST searching. The expressions of MHC and CFL2 protein were up-regulated remarkably, whereas the expression of 2-Cys Prxs was down-regulated remarkably, suggesting the anti-apoptosis ability of the low-temperature breeding group is strengthed.

Key words: Pseudosciaena crocea, Low temperature breeding, Liver, Two-dimentionlal gel electrophoresis(2-DE)