| 摘要: |
| 取喂食东海海参 60d 的 ICR 小鼠肝脏, 制备微阵列基因表达芯片, 并与模式小鼠芯片进行杂交, 筛选功能基因和差异表达蛋白。从食用海参的鼠肝中共筛选出了 4180 个基因, 其中差异表达基因 184 个, 上调基因 107 个, 下调基因 77 个。 利用 MAS.2.0 系统对其功能进行分析, 结果显示, 分子功能上有催化活性的基因占 39.86%, 结合功能的基因占 30.1%, 信号转导、分子转导、电子传递、分子马达等功能基因占 22.1%。 从生化过程分析可见与新陈代谢有关的基因 67.4%, 调控基因 16.2%, 发育等相关基因 9.1%。从细胞组成元件可见, 各种细胞成分为 51%, 细胞器为 21.6%, 其差异表达蛋白均为参与细胞信号传导的因子、合成及分解代谢的酶和供能物质。
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| 关键词: 东海海参, 微阵列芯片, 差异表达基因, 聚类分析 |
| DOI：10.11693/hyhz201101011011 |
| 分类号: |
| 基金项目:国家农业科技成果转化资金资助项目, 2007GB2C220359 号; 浙江省重大科技专项(优先主题)重大农业资助项目, 2008C02009-Ⅱ-2 号; 浙江省重大科技攻关资助项目, 2009C03017-4 号; 宁波市科技成果转化资金资助项目, 2007C100055 号; 宁波市国际合作资助项目, 2008C500265 号。 |
附件 |
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| STUDY ON THE PHYSIOLOGICAL FUNCTIONS OF ACAUDINA LEUCOPROCTA BY MICROARRAY |
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SU Xiu-Rong, JIN Chun-Hua, LI Yan-Yan, ZHOU Jun, DONG Ming-Min, ZHANG Chun-Dan, WANG Qi
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Faculty of Life Science and Biotechnology, Ningbo University
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| Abstract: |
| In order to make full use of sea cucumber Acaudina leucoprocta nutritional and health care values, this paper reported on screening differentially expressed genes of mice fed on sea cucumber using microarray hybridization. The results showed that a total of 4180 genes were screened from livers of mice fed with sea cucumber, of which 184 differentially expressed genes were found, 107 genes were found up-regulated and 77 genes were found down-regulated. Using MAS.2.0 to analyze molecular function of cluster, results showed that 39.86% of genes were with catalytic activities, genes with combinational function accounted for 30.1%, signal transduction, molecular transduction, electron transfer, molecular motors, and other functional genes accounted for 22.1%. Bio-chemical process analysis showed 67.4% of genes were with biochemical metabolic function, 16.2% were regulatory genes, and genes related to development were accounted for 9.1%. Analysis based on cell components, 51% of genes were responsible for making different components of cell, 21.6% were for organelles, proteins and other macromolecular complexes were 12.4%, 4.6% was for extracellular matrix, and 5.2% was for other small particulate matter. The differentially expressed proteins from the consumption of sea cucumber are involved in cell signaling factors, synthesis, catabolism enzymes and for the energetic materials. Therefore, long-term consumption of sea cucumbers can improve immunity, anti-fatigue, and anti-oxidation.
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| Key words: Acaudina leucoprocta, Microarray, Differentially expressed gene, Cluster analysis |
