| 摘要: |
| 利用线粒体细胞色素 c 氧化酶Ⅰ亚基(COⅠ)序列研究了南海海区鲻鱼群体的遗传多样性水平和遗传结构。通过 PCR 扩增与序列测定获得了长度为 621bp 的基因片段。在 35 个样品中共发现47 处碱基变异, A、T、G、C 碱基的平均含量分别为 31.6%、24.1%、25.0%和 19.2%。35 条 COⅠ序列共定义了 25 个单倍型, 存在 47 个多态性位点, 产生 49 个突变。其中, 简约信息位点有 36 个, 占总位点数的 5.8%, 同义替换位点 7 个, 占总变异位点的 14.9%, 没有发现插入或缺失现象。 单倍型多样性(Hd)为 0.9563, 核苷酸多样性(Pi)为 0.02795, 平均碱基差异(K)为 17.36。Tajima’s D 中性检验结果为 1.29916, 但差异不显著(P>0.10)。采用邻接法(NJ)和非加权配对算术平均法(UPGMA)构建分子系统树, 所有的 25 个单倍型被分成两个分支。结果表明, 南海海区鲻鱼种群具有较高的遗传多样性水平。其结果可以为合理开发和利用鲻鱼野生资源, 以及建立和保护鲻鱼种质资源提供必要的参考。
|
| 关键词: 鲻鱼, 线粒体 DNA, 细胞色素 c 氧化酶亚基Ⅰ(COⅠ), 遗传多样性 |
| DOI：10.11693/hyhz201101020020 |
| 分类号: |
| 基金项目:中央级公益性科研院所基本科研业务费项目, 东 2009M05 号, 东 2008M14 号, 东 2009M08 号 |
附件 |
|
| GENETIC DIVERSITY OF MUGIL CEPHALUS IN THE SOUTH CHINA SEA ANALYZED BY MITOCHONDRIAL COⅠ SEQUENCES |
|
SUN Peng, PENG Shi-Ming, YIN Fei, SHI Zhao-Hong
|
|
Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
|
| Abstract: |
| Genetic diversity and population structure of Mugil cephalus in the South China Sea were evaluated using a 621-base pair fragment of the mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene. A total of 47 polymorphic sites defined 25 distinct haplotypes from 35 samples. The average content of A, T, G and C was 31.6%, 24.1%, 25.0%, and 19.2%, respectively. The mean haplotype diversity (Hd), nucleotide diversity (Pi), and the average number of nucleotide difference (K) in the Mugil cephalus population were 0.9563, 0.02795, and 17.36, respectively. In all polymorphic sites, 36 parsimony-informative sites (5.8%), 7 synonymous substitutions (14.9%), and none of insertions and deletions were found. Result from Tajima’s D neutrality test for 25 haplotypes was 1.29916 (P>0.10). Neighbor-joining (NJ) phylogenetic tree and the Unweighted Pair Group Method with Arithmetic (UPGMA) phylogenetic tree divided 25 haplotypes into two clades. The present results revealed that the South China Sea population of Mugil cephalus had a high level of genetic diversity and no range expansion. These results provide basic information for the conservation and sustainable exploitation of this species.
|
| Key words: Mugil cephalus, Mitochondrial DNA, Cytochrome c oxidase subunitⅠ(COⅠ), Genetic diversity |
