| 引用本文: | 陈晓燕,温海深,何 峰,李吉方,陈彩芳,张葭人,金国雄,史 宝,史 丹,杨艳平.半滑舌鳎(Cynoglossus semilaevis)促性腺激素受体在雄性生殖周期中的表达.海洋与湖沼,2011,42(2):201-206. |
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| 摘要: |
| 采用RT-PCR方法, 研究了促性腺激素受体(FSHR和LHR)基因在雄性半滑舌鳎繁殖周期中的季节表达规律。结果表明, FSHR mRNA 在雄鱼精巢、脑和脾中及 LHR mRNA 在雄鱼的精巢和脾中均呈季节规律性变化。3 月份样品中, FSHR mRNA 在精巢表达量最低, 12 月份, 表达量最高; FSHR mRNA在脑中表达模式与精巢相反, 3月份表达量最高, 12月份达到最低值; FSHR mRNA在脾中表达模式和精巢相同。在 6 月份精巢样品中, LHR mRNA 表达量最低, 12 月表达量最高; 在脾中, LHR mRNA 在 3 月份表达量最低, 12 月份表达量最高。在精巢中, FSHR mRNA 和 LHR mRNA 都是在排精时表达量达到最大值, 说明这两种受体在雄性半滑舌鳎排精时发挥重要作用。 |
| 关键词: 半滑舌鳎, FSHR, LHR, 基因表达, 精巢, 脑, 脾 |
| DOI：10.11693/hyhz201102006006 |
| 分类号: |
| 基金项目:国家“十一五”科技支撑计划资助课题资助, 2006BAD09A01 号 |
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| EXPRESSION ANALYSIS OF FSHR AND LHR mRNAs DURING THE REPRODUCTIVE CYCLE OF MALES CYNOGLOSSUS SEMILAEVIS GüNTHER |
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CHEN Xiao-Yan, WEN Hai-Shen, HE Feng, LI Ji-Fang, CHEN Cai-Fang, ZHANG Jia-Ren, JIN Guo-Xiong, SHI Bao, SHI Dan, YANG Yan-Ping
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Key Laboratory of Mariculture, Ministry of Education, Ocean University of China
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| Abstract: |
| This paper focus on the study of the seasonal expression changes of gonadotropin receptors (FSHR and LHR) in reproductive cycle of male Half-smooth tongue sole using reverse-transcriptional polymerase chain reaction (RT-PCR). The results showed there were seasonal changes of expression of FSHR in testis, brain, and spleen and expression of LHR in testis and spleen. In testis, FSHR transcripts expressed at the lowest level in March and the highest in December. Interestingly, a reverse FSHR transcripts expression profiles was found for brain, the highest in March and the lowest in December. And there was a similar expression pattern for FSHR transcripts expressed in spleen and testis. For LHR transcripts, the highest level was found in December and the lowest in June for testis; the lowest level was found in March and the highest in December for spleen. As a whole, the transcript levels of FSHR and LHR at the highest during sperming, and these indicate that both kinds of gonadotropin receptors play important roles at sperming of male Half-smooth tongue sole. |
| Key words: Cynoglossus semilaevis Günther, Follicle-stimulating hormone receptor (FSHR), Luteinizing hormone receptor (LHR ), Gene expression, Testis, Brain, Spleen |
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