| 摘要: |
| 采用单因子实验设计方法, 进行了水体汞(Hg2+)在不同暴露时间对中华绒螯蟹血淋巴超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)和过氧化氢酶(CAT)活力以及脂质过氧化产物丙二醛(MDA)含量的影响研究。 结果表明, 较低浓度Hg2+(0.01和0.05mg/L)处理组中华绒螯蟹血淋巴SOD、GPX、CAT 活力在短时间内上升, 表现出一种“毒物兴奋效应”; 随着暴露时间的延长, 三种酶酶活力均受到明显的抑制; 高浓度的汞(0.20 和 0. |
| 关键词: Hg2+, 中华绒螯蟹, 血淋巴, SOD, GPX, CAT, MDA |
| DOI：10.11693/hyhz201103016016 |
| 分类号: |
| 基金项目:国家水体污染控制与治理科技重大专项资助项目, 2009ZX07528-002 号; 中国环境科学研究院中央级公益性科研院所基本科研业务专项资助, 2007KYYW08 号 |
附件 |
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| EFFECTS OF Hg2+ ON ANTIOXIDANT ENZYMATIC ACTIVITIES AND MALONDIADEHYDE CONTENT IN THE HAEMOLYMPH OF CHINESE MITTEN CRAB ERIOCHEIR SINENSIS |
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ZHAO Yan-Min1,2, WANG Xin-Hua3, QIN Yan-Wen1,2, ZHENG Bing-Hui1,2
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1.National Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences;2.State Environmental Protection Key Laboratory of Estuary and Coastal Environment, Chinese Research Academy of Environmental Sciences;3.College of Life Sciences, Nankai University
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| Abstract: |
| The effects of mercury (Hg2+) on antioxidant enzymatic activity and MDA content in haemolymph of juvenile Chinese mitten crab, Eriocheir sinensis, were investigated. The results showed significantly increased in activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) in the groups treated with 0.01 and 0.05mg/L Hg2+ in a short period of time, which appeared as “Poison-intoxication effect”; however, these antioxidant enzymes decreased in activities with increasing exposure time. In contrast, we found decreased enzyme activities in the presence of 0.20, and 0.30mg/L Hg2+ regardless on the length of the exposure time. MDA content in haemolymph of crabs exposed to different Hg2+ concentrations were significantly higher than controls, which appeared as dose and time dependable. Two ways analysis of variance was determined and results showed that both concentration and exposure time of Hg2+ to these juvenile Chinese mitten crabs had significant effects on these three antioxidant enzymes activities and MDA content. Based on our study, SOD, GPX, and CAT activities and MDA content in haemolymph of E. sinensis were sensitive to ambient Hg2+ and could be used as biomarkers in evaluating the toxicity of mercury or any other heavy metals contamination to crustacean. |
| Key words: Hg2+, Eriocheir sinensis, Haemolymph, SOD, GPX, CAT, MDA |
