| 摘要: |
| 模拟生产培育条件, 对条斑紫菜选育品系(T-17)和野生型品系(WT)的贝壳丝状体在不同温度下的壳孢子放散量及壳孢子萌发体的生长发育进行了研究。 结果表明, 在 18、 23、 24 和 25℃下, 连续放散16d的平均每壳的壳孢子量, T-17分别为8.6×106、 3.4×106、 5.4×105和0个, WT分别为8.0×106、2.8×106 |
| 关键词: 条斑紫菜, 耐高温性, 贝壳丝状体, 放散量, 壳孢子, 成活率 |
| DOI：10.11693/hyhz201103020020 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863计划)资助项目, 2006AA10A413号; 国家自然科学基金资助项目, 30571443号; 上海市科委重点项目, 10391901100 号; 上海市教委重点学科建设项目, J50701 号; 农业部公益性专项, 200903030-C 号 |
附件 |
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| THE RELEASING AND HIGH-TEMPERATURE RESISTANCE OF CONCHOSPORES FROM AN IMPROVED STRAIN IN PORPHYRA YEZOENSIS UEDA (BANGIALES, RHODOPHYTA) |
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FU Chun-Hui, YAN Xing-Hong, HUANG Lin-Bin, LI Lin
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College of Fisheries and Life Science, Shanghai Ocean University
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| Abstract: |
| The releasing, high-temperature resistance, and germination of the conchospores from an improved strain (T-17) of Porphyra yezoensis Ueda were studied. The average numbers of the total conchospores obtained from T-17 per shell over a releasing period of 16 days were 8.6×106, 3.4×106, 5.4×105, and 0 at 18, 23, 24, and 25℃, whereas were 8.0×106, 2.8×106, 4.9×105, and 0 for WT, respectively, There was no significant difference in the total numbers of conchospores between these two strains (P>0.05). The conchospores of both strains, being released at 18℃, all grew normally. When the conchospores released at 23℃, were cultured at three different high-temperatures (e.g. 23℃, 25℃ after a 3-day pre-culture at 24℃, or 25℃) for 16 days, the survival rates for T-17 were 84.6, 59.7, and 45.5%, whereas were 33.0, 20.9 and 17.1% for WT, respectively. The conchospore germlings of WT in these three culture conditions were abnormal in shape, and some rotted, while the conchospores of T-17 that were released and cultured at 23℃ grew into germlings in normal morphology, while under the other two culture conditions, the germlings grew irregularly. All the results indicate that the T-17 strain could release enough conchospores for cultivation, and its conchospores are tolerant to high-temperatures and can germinate normally into blades at 23℃, suggesting that the strain may offer broad application in the nori industry. |
| Key words: Porphyra yezoensis, High-temperature resistance, Conchocelis, Conchospore release, Conchospore, Survival rate |
