引用本文:	葛建,李明揆,林芳,赵进,韩宝瑜.PAEs 类环境激素在草鱼(Ctenopharyngodon idellus)脏器中代谢研究.海洋与湖沼,2011,42(4):549-553.

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*PAEs 类环境激素在草鱼(Ctenopharyngodon idellus)脏器中代谢研究*
葛建^1,2, 李明揆¹, 林芳¹, 赵进¹, 韩宝瑜²
1.中国计量学院生命科学学院;2.浙江省生物计量及检验检疫技术重点实验室

摘要:

利用 GC-FID 建立了草鱼血清、肝胰脏、小肠及肝细胞培养液中邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)、邻苯二甲酸丁基苄酯(BBP)和邻苯二甲酸二乙基己基酯(DEHP)同时检测方法, 并利用 LC-MS/MS 方法进行了 DEP、DBP、BBP 及 DEHP 在草鱼脏器中的代谢规律研究, 以揭示邻苯二甲酸酯类(Phthalic Acid Esters, PAEs)化合物在草鱼体内的致毒机制。结果表明, DEP、DBP、BBP 和 DEHP 在草鱼肝细胞中未见明显代谢, 但在草鱼血清、肝胰脏匀浆液和小肠匀浆液中均出现不同程度的代谢, 其中血清代谢较迅速, 其主要代谢产物为相应单酯类化合物, 包括邻苯二甲酸单乙酯(mEP)、邻苯二甲酸单丁酯(mBP)、邻苯二甲酸单苄酯(mBzP)及邻苯二甲酸-单-乙基己基酯(mEHP)。由此说明邻苯二甲酸酯类在草鱼脏器中主要被组织水解酶所水解, 血液和小肠是此类化合物经鳃和口染毒的第一代谢转化场所。

关键词: 草鱼, 邻苯二甲酸酯类, 气相色谱, 液相色谱-质谱联用

DOI：10.11693/hyhz201104013013

分类号:

基金项目:国家自然科学基金资助项目, 31100499 号; 浙江省自然科学基金资助项目, Y3080255 号、3100375 号; 浙江省分析测试计划项目, 2011C37044号

附件

METABOLISM OF PHTHALIC ACID ESTERS IN CTENOPHARYNGODON IDELLUS ORGANS AND HEPATOCYTE

GE Jian^1,2, LI Ming-Kui¹, LIN Fang¹, ZHAO Jin¹, HAN Bao-Yu²

1.College of Life Sciences, China Jiliang University;2.Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University

Abstract:

In order to reveal the toxicity mechanism of PAEs chemicals, the article developed a method for simultaneous determination of diethyl phthalate (DEP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBP) and di-(2-ethylhexyl) phthalate (DEHP) by using GC-FID. Meanwhile, the metabolism of four PAEs chemicals was also studied in Ctenopharyngodon idellus organs and hepatocyte through LC-MS/MS. The results showed that PAEs chemicals were not metabolized significantly in hepatocyte, but they were metabolized quickly in blood serum, intestinal tract and hepato-pancreas homogenate, especially in the serum. And the main metabolites were mono-phthalates, including monoethyl phthalate (mEP), monobutyl phthalate (mBP), monobenzyl phthalate (mBzP) and monoethylhexyl phthalate (mEHP). Therefore, it was concluded that the PAEs chemicals were mainly hydrolyzed by means of the proteolytic enzymes in C. idellus organs, and the blood and intestine were the first metabolized location of PAEs chemicals absorbed by the fish gill and intestine.

Key words: Ctenopharyngodon idellus, Phthalic acid esters, GC, LC-MS/MS