| 摘要: |
| 对发生在青岛近岸水域的后赤潮过程中赤潮藻密度、藻种进行鉴别的同时, 对现场水样的三维荧光光谱进行了测量, 获得了一套三维特征荧光数据。分析结果表明, 1)当海水中生物密度达到或接近赤潮密度时, Ex 最高峰值波长位于 245 nm, Em 最高峰值波长在 445nm, 激发转折波长在 260 nm, 荧光转折波长在 245 nm; 2)在非赤潮区, Ex 最高峰值波长要低于赤潮区的峰值波长, 约在 235 nm, Em最高峰值波长要稍高于赤潮区的荧光峰值波长, 激发转折波长和荧光转折波长也稍高于赤潮区的值; 3)当激发波长小于或等于 260 nm 时, 荧光波长大于激发波长的数值占总量的 84.78%; 大于260 nm, 荧光波长大于激发波长的数值占总量的 15.22%; 4)当激发波长为 410 nm, 荧光波长等于激发波长; 5)中肋骨条藻密度随特征荧光强度(Ex/Em)比值高度变化满足指数关系。 |
| 关键词: 后赤潮过程, 赤潮藻, 三维荧光光谱 |
| DOI：10.11693/hyhz201106023023 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 60578060 号。 |
附件 |
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| THREE-DIMENSIONAL EXCITATION EMISSION MATRIX ANALYSIS OF IN-SITU SEAWATER SAMPLES DURING THE TERMINATION STAGE OF A RED TIDE EVENT |
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WU Yong-Sen1, WU Shao-Yuan2, LI Yun3, LI Rui-Xiang1, YIN Yue-Fen1
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1.First Institute of oceanography;2.College of Physical and Envirmental Oceanography, Ocean University of China;3.College of Marine Life Sciences, Ocean University of China
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| Abstract: |
| While processing samples collected from coastal waters of Qingdao for algae cell density and species identification during the termination stage of a red tide event, we obtained a set of three-dimensional excitation emission matrix fluorescence spectra on filtered water samples. The results showed that 1) the wavelength with the highest excitation intensity was 245 nm, and the that with the highest emission intensity was 445 nm. The transitional wavelength for the excitation spectrum was 260 nm, and that for the emission spectrum was 245 nm when cell density in seawater was near or reached the density of typical red tide, 2) in the non-red tide area, the wavelength with the highest excitation intensity (235 nm) was lower than that in the red tide area, whereas the wavelength with the highest emission intensity was slightly higher, and the transitional excitation and emission fluorescent wavelengths in the non-red tide area were both higher than those in the red tide area, 3) when the excitation wavelength was less or equal to 260 nm, 84.78% of emission wavelengths were higher than the exciting wavelengths; and when the excitation wavelength was higher than 260nm, only 15.22% of emission wavelengths were higher than the exciting wavelengths, 4) when the excitation wavelength was 410 nm, the emission wavelength was equal to the excitation wavelength, 5) the density of Skeleconema costatum showed significant correlation with the exponential value of the excitation and emission intensity ratio. |
| Key words: Termination of red tide process, Density of red tide algae, Three-dimensional excitation-emission matrix fluorescence spectrum |
