引用本文:	郭宝英,祁鹏志,李继姬,叶莹莹,储张杰,吴常文.紫贻贝(Mytilus edulis)养殖群体的遗传多样性及遗传结构分析.海洋与湖沼,2012,43(1):52-56.

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*紫贻贝(Mytilus edulis)养殖群体的遗传多样性及遗传结构分析*
郭宝英¹, 祁鹏志², 李继姬¹, 叶莹莹¹, 储张杰¹, 吴常文¹
1.浙江海洋学院国家海洋设施养殖工程技术研究中心;2.华中农业大学水产学院

摘要:

基于线粒体 DNA 的 COⅢ基因序列对我国沿海重要经济贝类紫贻贝 3 个养殖群体(烟台、乳山、东极)的遗传多样性和遗传结构进行了研究。由 PCR 扩增获得 32 个体的 COⅢ基因 750bp 的部分序列, 其多态性遗传参数统计显示, 32个个体共检出18个单倍型, 总群体单倍型多样性指数(Hd)为 0.946, 核苷酸多样性指数(P_i)为 0.0207, 平均核苷酸差异数(K)为 15.316, 三个群体均显示出较丰富的遗传多样性。遗传分化系数(F_st)表明, 东极群体与烟台群体及乳山群体已明显分化, 而烟台群体和乳山群体间无遗传分化, 并且存在着较大的基因流。

关键词: 紫贻贝, COⅢ, 遗传多样性, 遗传结构

DOI：10.11693/hyhz201201008008

分类号:

基金项目:国家海洋公益性行业科研专项, 201005013 号; 国家国际科技合作项目, 2010DFA22770 号; 海洋渔业科学与技术浙江省重中之重学科开放课题, 20110218 号

附件

THE GENETIC DIVERSITY AND GENETIC STRUCTURE OF THREE CULTURED MYTILUS EDULIS POPULATIONS

GUO Bao-Ying¹, QI Peng-Zhi², LI Ji-Ji¹, YE Ying-Ying¹, CHU Zhang-Jie¹, WU Chang-Wen¹

1.Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;2.College of Fisheries, Huazhong Agricultural University

Abstract:

The genetic diversity and genetic structure of three cultured populations (Yantai of Shandong province, Rushan of Shandong province and Dongji of Zhejiang province) of Mytilus edulis were determined using the mitochondrial gene sequencing technology (COⅢ). The result revealed 18 haplotypes for total 32 individuals obtained using PCR method. The haplotype diversities (Hd), nucleotide diversities (P_i) and the average nucleotide differences (K) were 0.946, 0.0207 and 15.316, respectively, which showed high genetic diversity among populations. The fixation indices (F_st) of three populations showed that there was no genetic differentiation between Yantai and Rushan populations and significant genetic differentiation between Dongji and the other two populations.

Key words: Mytilus edulis, COⅢ, Genetic diversity, Genetic structure