| 摘要: |
| 以大海马幼体为实验材料, 通过设置不同的温度突变组(温度从 23℃突变至 15℃、28℃和33℃)的方法, 对其生长、生化组分以及酶活力的影响进行了研究。结果表明, 实验结束后, 28℃温度组的大海马幼体生长指标、蛋白含量、能值显著高于 23℃对照组(P<0.05), 而 15℃、33℃温度组的各项指标则显著低于对照组(P<0.05); 此外, 温度突变组的大海马幼体的酶活力均有先升后降的趋势, 在 1d 后出现峰值, 4—6d 各个温度组趋于稳定, 到实验第 15 天时, 28℃温度组的 SOD、ACP 活力和 MDA 的含量已处于 23℃对照组水平(P>0.05), CAT、AKP 活力显著高于 23℃对照组(P<0.05)。而 15℃、 33℃温度组的 SOD、 CAT 活力降至低于 23℃对照组水平(P<0.05), 15℃温度组的 ACP、 AKP活力则低于 23℃对照组水平(P<0.05), MDA 的含量在 15℃、33℃温度组随时间延长而增加。 |
| 关键词: 温度, 大海马, 生长, 活力 |
| DOI：10.11693/hyhz201201011011 |
| 分类号: |
| 基金项目:宁波市科技局项目, 2010C10055 号; 浙江省钱江人才计划项目, 2009R10012 号; 宁波市种子种苗工程项目, 2009040 号 |
附件 |
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| EFFECT OF TEMPERATURE MUTATIONS ON THE GROWTH, BODY COMPOSITION AND ENZYME ACTIVITIES OF JUVENILE HIPPOCAMPUS KUDA |
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SUN Bin1, CHEN Shun2, XU Yong-Jian1, DAI Guang-Pu1
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1.School of Marine Sciences, Ministry of Education Key Laboratory of Applied Marine Biotechnology, Ningbo University;2.Pingyang Marine and Fishery Bureau
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| Abstract: |
| The effects of variable temperature mutations (temperatures 23℃ dropped rapidly to 15℃, 28℃, 33℃) on the growth, biochemical composition and enzyme activities in juvenile Hippocampus kuda were studied. The results showed that growth index, protein content, energy value of H. kuda in 28℃ were significantly higher than the control group which was cultured in 28℃ (P<0.05) after the end of the experiment. However, the indicators of H. kuda in 15℃, 33℃ group was significantly lower than that of the control group (P<0.05), besides, the effect of variable temperature mutations groups on enzyme activities raised at first and then decreased, peaked after 1d, and leveled off after 4—6d. In the 15 days, the SOD, ACP activities and MDA content of 28℃group had no significantly difference with the control group (P>0.05), the CAT and AKP activity were apparently higher than that of the 23℃group (P<0.05). However, the SOD, CAT activities of 15℃, 33℃groups were apparently lower than that of 23℃ group (P<0.05). The ACP, AKP activities of 15℃ group had significantly lower than that of the control group (P<0.05), meanwhile, the MDA content of 15℃, 33℃ groups have increased with time elapse. |
| Key words: Temperature, Hippocampus kuda, Growth, Activity |
