| 摘要: |
| 以深海热液区化能自养菌Caminibacter profundus为对象, 采用扫描电镜和化学能谱分析, 研究了菌株表面形态和矿物元素沉积; 同时研究了菌株生长、甲基紫精(MV)还原的氢酶活性及膜结合的类型I NiFe 氢酶基因( hynL) 表达对盐度、pH 和温度几种环境因子变化的响应特点。结果表明, 菌株表面被Si 、Ca、S 和Fe等多种元素组成的矿物质层所包裹。不同条件下, 菌株生长、MV还原的氢酶活性和hynL的表达趋势相一致, 其最适条件为盐度30、pH 5.5和55℃培养温度。研究结果表明, 在环境改变时, C. profundus通过调控hynL的表达, 以调整菌株的能量代谢, 维系菌株的生长繁殖。 |
| 关键词: 深海热液区, 化能自养菌, Caminibacter profundus, 氢酶, 环境因子 |
| DOI：10.11693/hyhz201202012012 |
| 分类号: |
| 基金项目:中国大洋矿产资源研究开发协会资助项目, DYXM-115-02-2-19 号; 中央级公益性科研院所基本科研业务费专项资金项目, 2011T04 号 |
附件 |
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| CHARACTERISTICS OF HYDROGENASE FROM DEEP-SEA HYDROTHERMAL CHEMOAUTOTROPHIC CAMINIBACTER PROFUNDUS IN RESPONSE TO ENVIRONMENTAL FACTORS |
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HE Pei-Qing1, YUE Wen-Juan1,2, HUANG Xiao-Hang1
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1.Key Laboratory of Marine Bioactive Substance, the First Institute of Oceanography, State Oceanic Administration;2.Shandong Provincial Key Laboratory of Microbial Engineering , School of Food and Bioengineering
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| Abstract: |
| Hydrogenases play important roles in material and energy transfer in deep-sea hydrothermal microbial eco-system. In this paper, chemolithoautotrophic Caminibacter profundus in deep-sea hydrothermal was studied. The surface morphology and mineral deposition of the strain were analyzed by scanning electron microscopy and chemical spectrum. The bacterial growth, methyl viologen (MV)-reducing hydrogenase ac tivities and gene expres sions of membrane-bound type I NiFe hydrogenase large subunit ( hynL) in response to several environmental factors as salinity, pH and temperature, were also studied. The results showed that the strain surface was covered with minerals layers composed of Si, Ca, S, Fe, and other elements. The strains growth, MV-reducing hydrogenase activity demonstrated similar patterns with hynL gene expression under different conditions. The optimum conditions were salinity 30, pH 5.5 and 55℃ culture temperature. The results suggested that C. profundus can regulate hynL expression in response to the changes of environment to adjust metabolic energy, as well as the growth and propagation. |
| Key words: Deep-sea hydrothermal, Chemolithoautotrophic, Caminibacter profundus, Hydrogenase, Environ-mental factors |
