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引用本文:霍礼辉,陈彩芳,林志华,董迎辉,顾海龙,汪 青,包永波.镉诱导缢蛏(Sinonovacula constricta)体内金属硫蛋白基因变化规律研究.海洋与湖沼,2012,43(4):723-728.
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镉诱导缢蛏(Sinonovacula constricta)体内金属硫蛋白基因变化规律研究
霍礼辉1,2, 陈彩芳1, 林志华1, 董迎辉1, 顾海龙1,3, 汪 青1,3, 包永波1
1.浙江万里学院生物与环境学院;2.宁波大学海洋学院;3.上海海洋大学水产与生命学院
摘要:
采用荧光定量PCR法, 研究了缢蛏体内7种组织中金属硫蛋白(MT) mRNA的组织特异性表达, 以及不同浓度梯度重金属Cd2+对缢蛏内脏团中MT mRNA的诱导效应。结果表明, 在自然状态下, MT mRNA 在缢蛏内脏团中的相对表达量最高; 在不同浓度重金属Cd2+胁迫条件下, 缢蛏内脏团中MT mRNA的诱导表达具有明显的剂量效应和时间效应, 即随着Cd2+浓度的增加, MT mRNA的最大表达量明显升高, 而随着时间的变化, MT 基因的表达呈现脉冲式波动, 具有较强的时间依赖性。在3h和48h缢蛏内脏团中MT基因表达量与重金属Cd2+浓度之间具有较好的线性相关性, 说明缢蛏内脏团中MT mRNA表达变化可以作为指示环境重金属Cd2+污染的一个生物参考指标。
关键词:  缢蛏, Cd2+, MT mRNA, 内脏团
DOI:10.11693/hyhz201204006006
分类号:
基金项目:国家现代贝类产业技术体系项目资助, CARS-48号; 宁波市科技局重点科技项目, 2011C11004号; 宁波市科技创新团队项目, 2011B82017 号; 浙江万里学院省重中之重学科开放基金, KF2011005 号
附件
THE METALLOTHIONEIN GENE EXPRESSION IN SINONOVACULA CONSTRICTA EXPOSING TO CADMIUM
HUO Li-Hui1,2, CHEN Cai-Fang1, LIN Zhi-Hua1, DONG Ying-Hui1, GU Hai-Long1,3, WANG Qing1,3, BAO Yong-Bo1
1.College of Biological and Environmental Sciences, Zhejiang Wanli University;2.School of Marine Sciences, Ningbo University;3.College of Fisheries and Life Sciences, Shanghai Ocean University
Abstract:
The tissue specific expression of MT mRNA in seven tissues of Sinonovacula constricta and the effect of cadmium on visceral mass were studied using real-time quantitative PCR. In the absence of Cd2+, the level of MT mRNA was highest in visceral mass. But in the presence of Cd2+, the MT mRNA expression level in visceral mass of S. constricta showed significant dose and time dependency. With the increase of Cd2+ concentration, the level of MT expression increased significantly. The “pulse-scheme” expression showed by the time change indicated that MT-mRNA expression following metal exposure was largely dependent of time. Linear relationship existed between MT expression and Cd2+ concentration from 3h point and 48h point. The change of MT mRNA expression in visceral mass could be a biomarker index for Cd2+ pollution.
Key words:  Sinonovacula constricta, Cadmium, MT mRNA, Visceral mass
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