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引用本文:李 晔,陈 蕾,李 振,苏秀榕,周 君,李太武,江锦坡.浙江枝吻纽虫(Dendrorhynchus zhejiangensis)凝溶胶蛋白基因的原核表达产物抗肿瘤活性的研究.海洋与湖沼,2012,43(4):756-760.
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浙江枝吻纽虫(Dendrorhynchus zhejiangensis)凝溶胶蛋白基因的原核表达产物抗肿瘤活性的研究
李 晔1, 陈 蕾1, 李 振1, 苏秀榕1, 周 君1, 李太武2, 江锦坡2
1.宁波大学海洋学院;2.宁波城市职业技术学院
摘要:
凝溶胶蛋白(Gelsolin)作为一种重要的肌动蛋白调节蛋白, 在机体的多种生理和病理活动中发挥重要作用。本文采用细胞MTT染色法, Hoechst33342/PI 荧光染色和流式细胞仪技术, 对浙江枝吻纽虫(Dendrorhynchus zhejiangensis)的gelsolin基因的原核表达产物(r-gelsolin)进行了体外抗肿瘤活性的研究。结果表明, 30μg/ml的r-gelsolin作用于胃低分化粘液腺癌MGC803细胞24h后, 细胞的增殖抑制率达到最高, 约为34.53%。荧光染色显示, r-gelsolin 使MGC803细胞的正常形态发生改变, 细胞核出现核固缩, 破裂, 产生凋亡小体等现象。进一步通过流式细胞仪证实, r-gelsolin 可以阻滞MGC803细胞于S、G2期, 影响细胞分裂的进程, 并促使细胞发生凋亡。
关键词:  浙江枝吻纽虫, 凝溶胶蛋白, 胃癌细胞, 细胞凋亡
DOI:10.11693/hyhz201204011011
分类号:
基金项目:国家自然科学基金项目, 31272637号; 浙江省自然科学基金资助项目, Y3100480 号; 浙江省教育厅(理), Y200907951号
附件
THE ANTI-TUMOR ACTIVITIES OF PROKARYOTIC RECOMBINANT EXPRESSION PRODUCT OF GELSOLIN GENE FROM DENDRORHYNCHUS ZHEJIANGENSIS
LI Ye1, CHEN Lei1, LI Zhen1, SU Xiu-Rong1, ZHOU Jun1, LI Tai-Wu2, JIANG Jin-Po2
1.School of Marine Science, Ningbo University;2.Ningbo City Collage of Vocational Technology
Abstract:
Gelsolin is an important actin binding protein and involved in many cellular processes. In this work, anti-tumor activities of the prokaryotic recombinant expression product of gelsolin gene from Dendrorhynchus zhejiangensis were assessed employing the MTT assay, Hoechst33342/PI staining and a flow cytometric analysis. The results showed that the growth inhibition rate of MGC803 cells was reached to 34.53% when the cells were treated by 30μg/ml r-gelsolin for 24h. Effects of r-gelsolin on the morphology of MGC803 cells were observed by Hoechst33342/PI staining. The cell nuclei showed pyknosis, rupture, and apoptotic bodies appeared. Furthermore, cell cycle and apoptosis were detected by flow cytometer, compared with the control group, the results showed that r-gelsolin could arrest the cells in S/G2 phase, inhibit the proliferation of cells, and induce their apoptosis.
Key words:  Dendrorhynchus zhejiangensis, Gelsolin, Gastric cancer cells, Cell apoptosis
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