引用本文:	张飞,苏永全,王军,覃映雪,马英,鄢庆枇.大黄鱼(Pseudosciaena crocea)源美人鱼发光杆菌(Photobacterium damselae)的分离鉴定及致病性研究.海洋与湖沼,2012,43(6):1202-1208.

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*大黄鱼(Pseudosciaena crocea)源美人鱼发光杆菌(Photobacterium damselae)的分离鉴定及致病性研究*
张飞¹, 苏永全², 王军², 覃映雪¹, 马英¹, 鄢庆枇¹
1.集美大学水产学院;2.厦门大学海洋与地球学院

摘要:

于2010年从宁德患病大黄鱼中分离得到一株优势菌NPL1006, 经鉴定该菌株为美人鱼发光杆菌。人工感染实验结果显示该菌对大黄鱼的半致死浓度(LD₅₀)为7.96×10³CFU/g体重。药敏性实验结果显示NPL1006对头孢唑啉、头孢噻吩和诺氟沙星等9种药物高度敏感。采用纸片法检测得到NPL1006的胞外产物具有蛋白酶、明胶酶和淀粉酶活性。胞外产物感染斑马鱼研究结果显示其LD₅₀为1.20mg蛋白/kg体重。以上结果表明大黄鱼源美人鱼发光杆菌NPL1006胞外产物含有多种毒力因子, 与该菌对大黄鱼的致病性密切相关。

关键词: 大黄鱼, 美人鱼发光杆菌, 胞外产物, 致病性

DOI：10.11693/hyhz201206025025

分类号:

基金项目:公益性行业(农业)科研专项(200903029); 国家自然科学基金项目(31272699, 41176115); 福建省杰出青年基金项目(2011J06014)资助。

附件

STUDIES ON THE ISOLATION, IDENTIFICATION AND VIRULENCE OF PHOTOBACTERIUM DAMSELAE ISOLATED FROM PSEUDOSCIAENA CROCEA

ZHANG Fei¹, SU Yong-Quan², WANG Jun², QIN Ying-Xue¹, MA Ying¹, YAN Qing-P¹

1.Fisheries College of Jimei University;2.College of Ocean and Earth Sciences, Xiamen University

Abstract:

A dominant strain (NPL1006) was isolated from diseased Pseudosciaena crocea in 2010. The results of artificial infection experiment showed that the LD₅₀ of NPL1006 to P. crocea was 7.96×10³ CFU/g body weight. NPL1006 was identified as Photobacterium damselae by Time of Flight Mass Spectrometer and the 16S rDNA sequencing analysis. Susceptibility experiments showed that NPL1006 is highly sensitiveto 9 drugs such as Cefazolin, Cefalotin and Norfloxacin. The extracellular products of NPL1006 were found to exhibit protease, gelatinase and amylase activities. The LD₅₀ of extracellular products to zebrafish was 1.20mg protein/kg body weight. These results suggested thatextracellular products of P. damselae NPL1006 separated from P. crocea contained a variety of virulent factors, and it’s closely related to the pathogenicity of the bacteria P. crocea.

Key words: Pseudosciaena crocea, Photobacterium damselae, ECP, Pathogenicity