| 摘要: |
| 采用现场测定、磷脂脂肪酸(PLFA)法及基质脱氢酶活性测定等方法, 对4种不同养殖模式的凡纳滨对虾池塘的水质、沉积物酶活性及微生物群落结构进行了分析和研究。结果表明: 养殖末期, 凡纳滨对虾淡水混养和海水混养池塘水质较好, 海水集约化池塘水质最差; 其中海水集约化池塘沉积物基质中有机质含量、微生物生物量、微生物活性和酶活性都很高, 真菌、G+菌的相对含量则很低。主成分分析(PCA)表明: 第一主成分主要与脲酶、有机质含量、微生物活性、总PLFA、B/F有显著的正相关关系(r>0.9), 而与Gram+/Gram-、pH、18:1ω9C/T、单不饱和/支链却存在显著的负相关关系(r>0.9); 第二主成分主要与脱氢酶有显著正相关关系(r>0.8), 四种池塘在PCA图中分散开, 说明其微生物群落结构及环境状况存在明显差异。 |
| 关键词: 凡纳滨对虾, 沉积物, 酶活性, PLFA, 微生物群落结构 |
| DOI：10.11693/hyhz201206032032 |
| 分类号: |
| 基金项目:国家科技支撑计划项目, 2011BAD13B08 号; 宁波市海洋与渔业局项目, 2012-1-6 号; 宁波市自然科学基金项目, 2012A610168号。 |
附件 |
|
| STUDY ON MICROBIAL ENZYME ACTIVITY AND MICROBIAL COMMUNITY STRUCTURE IN THE SEDIMENT OF LITOPENAEUS VANNAMEI CULTURED PONDS WITH DIFFERENT MODELS |
|
LI Yue-Shu1, ZHENG Zhong-Ming1, ZHAI Hai-Jia1, HUA Jian-Quan2, ZHOU Chao-Sheng3, CHEN Hui-Huang1
|
|
1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;2.Promotion Center of Fisheries in Cixi City;3.Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource
|
| Abstract: |
| Water quality, physical-chemical factors, microbial enzymes activity, and microbial community structure were studied in last phase’s sediment of greenhouse intensive culture and ecological culture ponds for Litopenaeus vannamei from April to October in 2011 in Ningbo. The results showed that water quality in the ponds for ecological culture mode was better than that for intensive culture, and even more worst environment of seawater intensive ponds. Seawater intensive ponds had high value of organic matter, microbial biomass, microbial activity and enzymatic activity, and lower fungi and gram-positive bacteria biomass in sediment. Principal component analysis (PCA) showed that: the first principle component were significantly correlated with urease, organic matter content, microbial activity, B/F and total PLFA (r>0.9), but assumes the remarkable inverse correlation with Gram+/Gram-, pH, 18:1ω9C/T and single unsaturated/Branched chain (r>0.9). The second principle component were significantly correlated with dehydrogenase (r>0.8). The scattered points representing cultured ponds with different models showed significant differences in the PCA figure. |
| Key words: Litopenaeus vannamei, Sediment, Enzymatic activity, PLFA, Microbial community structure |
