| 摘要: |
| 以大菱鲆(Scophthalmus maximus)为实验材料, 研究其繁殖期内卵子和卵巢液中磷酸酶活性变化规律及其与受精率相关性。结果表明, 在大菱鲆繁殖周期内, 卵子碱性磷酸酶和酸性磷酸酶活性呈相反的变化趋势, 且排卵中期卵子碱性磷酸酶和酸性磷酸酶活性分别显著低于和高于早期和晚期(P<0.05), 而卵巢液中碱性磷酸酶和酸性磷酸酶活性呈同卵子中相反的变化趋势; 此外卵子碱性磷酸酶和酸性磷酸酶及卵巢液酸性磷酸酶活性同受精率显著相关(P<0.05)。以上结果表明, 大菱鲆卵子和卵巢液中碱性磷酸酶和酸性磷酸酶活性同受精率存在着显著相关性, 其活性变化在一定程度上反映卵子质量优劣, 可作为评判卵子质量辅助指标。 |
| 关键词: 大菱鲆 卵子 卵巢液 碱性磷酸酶 酸性磷酸酶 受精率 |
| DOI：10.11693/hyhz20120808002 |
| 分类号: |
| 基金项目:国家鲆鲽类产业技术体系建设高效养殖模式专项资金项目, CRAS-50 号; 国家自然科学基金, 31302205 号; 中国博士后基金, 2012M511559 号, 2013T60690 号; 山东省自然科学基金, ZR2012CQ024 号; 山东省优秀中青年科学家基金，BS2013SW004号。 |
附件 |
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| ACTIVITIES OF PHOSPHATASE IN EGGS AND OVARIAN FLUIDS AND ITS CORRELATION WITH THE FERTILIZATION RATE DURING THE REPRODUCTIVE CYCLE OF TURBOT (SCOPHTHALMUS MAXIMUS) |
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JIA Yu-Dong, MENG Zhen, LIU Xin-Fu, GAO Chun-Ren, NIU Hua-Xin, LEI Ji-Lin
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Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology
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| Abstract: |
| We investigated the activity and variation of phosphatase in eggs and ovarian fluids during the reproductive cycle of turbot (Scophthalmus maximusL.), and explored whether and how phosphatase activity is influenced by fertilization rate. Results of experiments demonstrate that the activity and variation of the alkaline phosphatase and acid phosphatase in eggs was opposite in general to those in ovarian fluid. In eggs, alkaline phosphatase activity in ovulated metaphase was significantly lower than those of the early and late stage of ovulation. In contrast, acid phosphatase activity was strikingly higher than those of the early and late stage of ovulation. In addition, we found that the alkaline phosphatase
activity in both eggs and ovarian fluid were closely related tofertilization rate, and acid phosphatase activity in ovarian fluid was much affected by fertilization rate. Therefore, activities of both alkaline and acid phosphatases in eggs and acid phosphatase activity in ovarian fluid were significantly related to fertilization rate, which may be applied to indicate the quality of turbot eggs. |
| Key words: turbot Scophthalmus maximus egg ovary fluids alkaline phosphatase acid phosphatase fertilization rate |
