| 摘要: |
| 为研究微藻来源的β-葡聚糖生物活性以及是否存在种间差异，从6种海洋硅藻和定鞭藻（Haptophyte）中制备了β-葡聚糖，进行了抗肿瘤、抗氧化、抑菌活性的分析和比较。6种微藻分别为：中肋骨条藻（Skeletonema costatum）、三角褐指藻（Phaeodactylum tricornutum）、假微型海链藻（Thalassiosira pseudonana）、柔弱角刺藻（Chaetoceros debilis）、球等鞭金藻（Isochrysis galbana）、颗石藻（Pleurochrysis sp.）。研究结果显示，1、微藻β-葡聚糖对Hela肿瘤细胞有细胞毒活性：浓度为50-100μg/ml时，假微型海链藻β-葡聚糖的活性最高；200μg/ml时，颗石藻的最高，但活性均未超过50%。2、对羟基自由基和DPPH自由基均有浓度相关的清除率。对羟基自由基清除率高于对DPPH自由基的作用。浓度为400μg/ml时，对羟基自由基清除率可达72%-74%。3、有浓度相关的抑制大肠杆菌的作用，浓度为400μg/ml时，抑菌率可达60%。有促进金黄色葡萄球菌生长，其中，中肋骨条藻的β-葡聚糖有浓度相关的作用。结果表明，几种硅藻和定鞭藻的β-葡聚糖生物活性功能相同，都具有抗肿瘤、抗氧化以及抑菌或促菌作用，活性大小与浓度有明显的相关性。相同浓度或作用时间下，其活性大小及活性饱和性具有微藻种间差异。这为研究和利用微藻及其天然产物提供了参考。 |
| 关键词: 海洋微藻 β-葡聚糖 抗肿瘤 抗氧化 抑菌活性 |
| DOI：10.11693/hyhz20121120001 |
| 分类号: |
| 基金项目:国家科技攻关计划 |
附件 |
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| Studies on bioactivities of β-glucan from marine microalgae |
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zhouchengxu,Tian Tian,Chen Haimin,Yan Xiaojun and Luo Qijun
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Ningbo University,Ningbo University,Ningbo University,Ningbo University,Ningbo University
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| Abstract: |
| The differences of the biological activity of β-glucans are significant in regards to different organisms.The bioacitivities, including cytotoxicity, antioxidation, and antimicrobial from β-glucan, which were prepared from mass cultures of six strains of marine diatom and haptophyte, have been studied. The six strains of marine microalgae include Skeletonema costatum, Phaeodactylum tricornutum, Thalassiosira pseudonana, Chaetoceros debilis, Isochrysis galbana and Pleurochrysis sp.. The results showed that (1) β-glucan from each microalgal strain has moderate cytotoxicity on Hela cells, with the highest value less than 50%, among which, the most potent β-glucan is from T. pseudonana, is at the concentration range from 50-100 μg/ml, and Pleurochrysis sp. at the concentration of 200 μg/ml. The β-glucan from I. galbana and Pleurochrysis sp. show best concentration-cytotoxcitiy coefficiency. (2) β-glucan from each microalgal strain has concentration relevant antioxidation on hydroxyl radical and DPPH, and more active on hydroxyl radical than on DPPH. At 400μg/ml, the highest values for hydroxyl radical are 72% to 74%. (3) The effects on microorganism had similar chacacteristics from the different strains of microalgae. Interestingly, β-glucan from the marine microalga can inhibit the growth of E. coli (the highest was 60% at the concentration of 400 μg/ml), and stimulate the growth of Staphylococcus aureus (most significant shown for β-glucan from S. costatum). It is demonstrated that, the biological activities of β-glucans of the 6 species of microalgae included cytotoxicity, antioxidation, and antimicrobial. The activities were concentration and time dependent. Some significant difference existed among the microalga when the glucan concentration was the same. |
| Key words: microalgae β-glucan cytotoxicity antioxidation antimicrobial |
