首页 | 期刊简介 | 编委会 | 投稿指南 | 常用下载 | 联系我们 | 期刊订阅 | In English
引用本文:黄鹤忠,梁建生,张群英.菊花江蓠(Gracilaria lichenoides)对N、P吸收效应及其细胞超微结构变化.海洋与湖沼,2013,44(1):95-102.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2251次   下载 2580 本文二维码信息
码上扫一扫!
分享到: 微信 更多
菊花江蓠(Gracilaria lichenoides)对N、P吸收效应及其细胞超微结构变化
黄鹤忠1,2, 梁建生1, 张群英2
1.扬州大学生物科学与技术学院;2.苏州大学基础医学与生物科学学院
摘要:
通过设置5个N/P浓度梯度和3个N浓度下的4个N∶P梯度, 进行了江蓠对N、P的短期(0—4h)和长期(第14天)吸收速率(SNURLNUR)以及短期和长期吸收效率(SNUELNUE)的研究, 同时观察了5个N/P浓度梯度对江蓠细胞超微结构的影响。结果表明: N∶P比为16∶1时, 江蓠对N、P的SNURLNUR均最大(P<0.05); N/P为1200/75和960/60μmol/L浓度时, 分别对N和P的SNUR最大, 达12.85和0.79μmol/(gDW·h), 但此N/P浓度下长期培养的江蓠叶绿体结构存在不同程度的损伤; 而N/P浓度480/30μmol/L时, 对N和P的LNUR均最大值4.23和0.31μmol/(gDW·h)。江蓠对N、P的SNUELNUE在介质N/P 60/3.75μmol/L时为最高, 而在1200/75μmol/L时为最低(P<0.05)。
关键词:  江蓠, 氮, 磷, 吸收效应, 超微结构
DOI:10.11693/hyhz201301015015
分类号:
基金项目:江苏省社会发展基金资助项目, BS2002016 号
附件
UPTAKE EFFECTS OF NITROGEN AND PHOSPHORUS AND CELL ULTRASTRUCTURE CHANGES OF GRACILARIA LICHENOIDES
HUANG He-Zhong1,2, LIANG Jian-Sheng1, ZHANG Qun-Ying2
1.College of Bioscience and Biotechnology, Yangzhou University;2.School of Basic Medicine and Biological Science, Soochow University
Abstract:
The effects of variable 5 N/P concentration levels and 4 N∶P ratio levels of 3 N concentration levels, respectively, on uptake rates (SNUR and LNUR) and uptake efficiency (SNUE and LNUE) of N (TIN) and P (PO3-4-P) by Gracilaria lichenoides for short-term (0—4h) and long-term (over a 24h period at 14th day) were studied. Meanwhile, ultrastructure of cells of G. lichenoides cultivated in different N and P concentration for 14 day was observed. The results showed that optimal N∶P ratio in culture media which SNUR and LNUR for N and P by G. lichenoides reached the highest value among different N/P concentrations (P<0.05) was 16∶1. The reached maximum SNUR of N and P by G. lichenoides was respectively 12.85 and 0.79μmol/(gDW·h), when the N/P concentration in medium was 1200/75 and 960/60μmol/L, but the ultrastructure of chloroplasts of G. lichenoides cultivated in those N/P concentration for 14 day emerged some extent damage. The reached maximum LNUR of N and P by G. lichenoides were 4.23 and 0.31μmol/(gDW·h), respectively, when the N/P concentration in medium was 480/30μmol/L. The reached maximum SNUE and LNUE of N and P by G. lichenoides was in the medium of N/P 60/3.75μmol/L, but decreased to the minimum SNUE and LNUE, respectively, was in the medium of N/P 1200/75μmol/L.
Key words:  Gracilaria lichenoides, Nitrogen, Phosphorus, Uptake effect, Ultrastructure
版权所有 海洋与湖沼 Oceanologia et Limnlolgia Sinica Copyright©2008 All Rights Reserved
主管单位:中国科协技术协会 主办单位:中国海洋湖沼学会
地址:青岛市海军路88号  邮编:266400  电话:0532-82898753  E-mail:ols@qdio.ac.cn
技术支持:北京勤云科技发展有限公司