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| 引用本文: | 范艳波,王中铎,郭昱嵩,颜云榕,刘楚吾.南海海域红斑后海螯虾(Metanephrops thomsoni)的分子识别与群体遗传分化.海洋与湖沼,2013,44(1):220-225. |
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| 南海海域红斑后海螯虾(Metanephrops thomsoni)的分子识别与群体遗传分化 |
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范艳波1, 王中铎1, 郭昱嵩1, 颜云榕1,2, 刘楚吾1
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1.广东海洋大学水产学院 南海水产经济动物增养殖广东普通高校重点实验室;2.广东海洋大学南海渔业资源监测与评估中心
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| 摘要: |
| 利用16S rRNA 基因序列分子识别红斑后海螯虾, 然后对海南附近海域(18°30′—19°00′N, 111°30′—112°30′E)、北部湾口海域(15°41′N, 110°40′E)和南沙群岛海域(5°20′—5°29′N, 110°09′—111°26′E)三个地理群体进行ITS1的扩增测序。16S rRNA基因序列的结果表明所取样本准确; 三个地理群体分别得到616—623bp、619bp和614bp的ITS1全长序列, A、G、T、C含量平均分别为22.3%、29.4%、17.8%和30.6%; 其中562个保守位点, 39 个多态位点。ML和NJ聚类分析显示三个地理群体共聚为三个大支, 其中海南群体有群体分化。表明红斑后海螯虾三个地理群体分化极其显著, 且海南群体具有相对高的遗传多样性。 |
| 关键词: 红斑后海螯虾, 16S rRNA, ITS1, 遗传多样性 |
| DOI：10.11693/hyhz201301033033 |
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| 基金项目:国家自然科学基金资助项目, 31101904 号, 31201996 号; 广东省科技计划项目资助, 2010B020308004 号 |
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| MOLECULAR RECOGNITION AND POPULATION GENETIC DIFFERENTIATION OF METANEPHROPS THOMSONI IN SOUTH CHINA SEA |
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FAN Yan-Bo1, WANG Zhong-Duo1, GUO Yu-Song1, YAN Yun-Rong1,2, LIU Chu-Wu1
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1.Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University;2.Center of South China Sea Fisheries Resources
Monitoring and Assessment, Guangdong Ocean University
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| Abstract: |
| The research on the clawed lobster species Metanephrops thomsoni, distributed in the coastal areas of China, Japan and Philippines, are rarely reported in the world. Regularly, the 16S ribosomal RNA sequences (16S rRNA) and the internal transcribed spacer 1 (ITS1) were used for the genetic diversity analysis and species identification. After recognized the M. thomsoni using 16S rRNA (GenBank Accession No.JQ229830), the length of ITS1 sequences from three geographic populations, Hainan (18°30′—19°00′N, 111°30′—112°30′E), the mouth of Beibu Gulf (15°41′N, 110°40′E) and Nansha Islands (5°20′—5°29′N, 110°09′—111°26′E) was 616—623bp, 619bp and 614bp, respectively. The average contents of A, G, T and C was 22.3%, 29.4%, 17.8% and 30.6% separately, and containing 562 conserved loci and 39 polymorphic loci in ITS1 sequences of these three populations. There were four Haplotypes (GenBank Accession No.JQ229831—JQ229834) and their statistical significance was P<0.02. The results of phylogenetic cladistics suggested that three different areas of M. thomsoni were classified into three branches based on Maximum Likelihood tree and Neighbor-joining tree, especially the significant differentiation was displayed in Hainan geographic populations. The analytical result of ITS1 sequences indicated that three geographic populations of M. thomsoni have significant differentiation and Hainan geographic population has large genetic diversity especially. |
| Key words: Metanephrops thomsoni, 16S rRNA, ITS1, Genetic diversity |
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