| 摘要: |
| 在水温(27.4±1.3)℃条件下, 以体长(33.23±1.66)mm、体质量(0.7893±0.1518)g的日本沼虾为实验动物, 采用静水停食法开展了Zn2+对日本沼虾的急性毒性实验, 并以此为基础, 测定了Zn2+不同质量浓度水平下日本沼虾的耗氧率与窒息点。结果表明: (1) Zn2+对日本沼虾的急性致死效应与其呼吸生理代谢受阻密切相关; (2) Zn2+对日本沼虾24h、48h、72h、96h的半致死质量浓度依次为1.493、0.844、0.643 和0.464mg/L; (3) Zn2+对日本沼虾昼均、夜均和日均耗氧率均无显著影响的阈值为0.046mg/L, 该值为Zn2+对日本沼虾急性毒性的安全质量浓度; (4) Zn2+对日本沼虾产生轻度胁迫和中度胁迫的质量浓度范围分别为0.046—0.064mg/L和0.064—0.082mg/L。 |
| 关键词: 日本沼虾, Zn2+, 半致死质量浓度, 耗氧率, 窒息点 |
| DOI：10.11693/hyhz201301035035 |
| 分类号: |
| 基金项目:浙江省重大科技专项农业重点项目, 2008C12083 号 |
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WANG Zhi-Zheng, REN Su-Yi, ZHAO Jing, FU Ying-Jie, YANG Peng
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Zhejiang Ocean University
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| Abstract: |
| The acute toxicity experiment of zinc on freshwater shrimp (Macrobrachium nipponensis), with (33.23± 1.66)mm total length and (0.7893±0.1518)g weight, was carried out in static water without feeding at the water temperature (27.4±1.3)℃. Based on this experiment, the oxygen consumption rate and the suffocation point of M. nipponensis exposed to different concentrations of zinc were measured. The result showed that there was a close relationship between the acute lethal effect of Zn2+ and the inhibition of respiratory metabolism. The LC50 of Zn2+on shrimp at 24, 48, 72 and 96h were 1.493, 0.844, 0.643, and 0.464mg/L, respectively. The threshold value of zinc concentration that causes no significant difference in the average oxygen consumption and suffocation point of M. nipponensis was 0.046mg/L, and this value was set as the safe concentration that zinc has no acute toxic effect on M. nipponensis. The mild stress range is from 0.046mg/L to 0.064mg/L and moderate stress range is from 0.064mg/L to 0.082mg/L for Zn2+ on M. nipponensis. |
| Key words: Macrobrachium nipponensis, Zn2+, LC50, Oxygen consumption rate, Suffocation point |
