| 引用本文: | 肖志忠,肖永双,任桂静,高天翔,涂登志,韩志强,马道远,徐世宏,刘清华,李 军.条石鲷(Oplegnathus fasciatus)养殖群体与野生群体线粒体控制区序列遗传变异研究.海洋与湖沼,2013,44(1):249-254. |
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| 条石鲷(Oplegnathus fasciatus)养殖群体与野生群体线粒体控制区序列遗传变异研究 |
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肖志忠1,2, 肖永双2, 任桂静3, 高天翔1, 涂登志1, 韩志强4, 马道远2, 徐世宏2, 刘清华2, 李 军2
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1.中国海洋大学 海水养殖教育部重点实验室;2.中国科学院海洋研究所;3.中国水产科学研究院东海水产研究所;4.浙江海洋学院
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| 摘要: |
| 采用线粒体(mtDNA)控制区序列分析方法, 进行了条石鲷养殖群体(F1代和F2代)与野生群体的遗传变异比较研究。结果表明: 在长度为484bp的mtDNA控制区片段中, 条石鲷养殖群体F1代单倍型多样度水平(h= 0.955±0.047)略低于野生群体(h= 1.000±0.034), 却显著高于养殖F2代群体(h= 0.797±0.070); 条石鲷野生群体(π = 0.021±0.012)、养殖群体F1代(π= 0.018±0.010)和养殖群体F2代(π = 0.014±0.008)的核苷酸多样度水平呈现明显的递减趋势; 核苷酸不配对分布和两两序列相比较碱基差异结果显示条石鲷野生群体、养殖群体F1代和养殖群体F2代也呈现明显的递减趋势。分子方差分析(AMOVA)、两两群体相比较的ФST和确切P检验结果皆显示条石鲷养殖群体F1代和F2代与野生群体存在显著的遗传分化。单倍型最小跨度分析和NJ系统分析结果均未检测到显著的谱系结构。 |
| 关键词: 条石鲷, 养殖群体, 野生群体, 线粒体控制区, 遗传变异 |
| DOI：10.11693/hyhz201301037037 |
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| 基金项目:“十二五”农村领域国家科技计划课题, 2012AA10A413-1 号; 中国科学院创新前沿项目, KSCX2-YW-N-47-08 号; 海洋渔业科学与技术浙江省重中之重学科开放课题, 20110208 号; 山东省优秀中青年科学家科研奖励基金课题, BS2012HZ025; 农业部东海与远洋渔业资源开发利用重点实验室开放课题, K201204 号; 国家2012 年度留学回国人员科技活动项目择优资助课题, 2013.01 |
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| COMPARATIVE ANALYSIS ON THE GENETIC VARIATION OF CULTURED AND WILD ROCK BREAM OPLEGNATHUS FASCIATUS POPULATION BASED ON mtDNA CONTROL REGION |
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XIAO Zhi-Zhong1,2, XIAO Zhi-Zhong2, XIAO Zhi-Zhong3, XIAO Zhi-Zhong1, TU Deng-Zhi1, HAN Zhi-Qiang4, MA Dao-Yuan2, XU Shi-Hong2, LIU Qing-Hua2, LI Jun2
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1.The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China;2.Institute of Oceanology, Chinese Academy of Sciences;3.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;4.Zhejiang Ocean University
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| Abstract: |
| Partial nucleotide sequences of mitochondrial DNA control region fragment were sequenced and analyzed to estimate genetic differentiations between cultured (F1and F2) and wild rock bream (Oplegnathus fasciatus) populations. The result showed that the haplotype diversity of cultured population (F1) (h= 0.955±0.047) was just a little below that of wild population (h= 1.000±0.034), but which was significantly higher than that of F2(h= 0.797±0.070) based on the length of 484bp sequence. Significant decline trend of nucleotide diversity was detected from wild population (π = 0.021± 0.012) to cultured population F1(π = 0.018±0.010) and F2(π = 0.014±0.008), the same results were also checked in the Mismatch analysis and average number of nucleotide differences analysis in the present study. Significant population genetic differentiations were detected between cultured population (F1 and F2) and wild population based on the analyses of AMOVA, conventional ΦST and the exact test. No significant genealogical structure corresponding to the cultured and wild populations was detected in the species based on the MST and NJ analyses. |
| Key words: Oplegnathus fasciatus, Cultured population, Wild population, mtDNA control region, Genetic variation |