| 摘要: |
| 采用解剖及石蜡切片技术, 观察研究了养殖光唇鱼(Acrossocheilus fasciatus)卵巢发育的形态及组织学结构特征。结果表明, 第一次性周期内, 4—5月龄鱼卵巢处Ⅰ期, 内以第Ⅰ时相卵细胞为主; 6—18月龄鱼卵巢处Ⅱ期, 内以第Ⅱ时相卵母细胞为主, 单层滤泡膜形成; 19—20月龄鱼卵巢处Ⅲ期, 内以第Ⅲ时相卵母细胞为主, 双层滤泡膜及放射带形成, 胞质内出现液泡及卵黄粒; 21—22月龄鱼卵巢处Ⅳ期, 内以第Ⅳ时相卵母细胞为主, 胞质内充满卵黄粒; 23—24月龄鱼卵巢处Ⅴ期, 内以第Ⅴ时相卵母细胞为主, 滤泡膜脱落; 25月龄鱼卵巢处Ⅵ期, 内以空滤泡和第Ⅱ、第Ⅲ时相卵母细胞为主。6—7月为主要产卵期, 9 月产后卵巢恢复至Ⅱ期, 12 月进入Ⅲ期, 并以该期越冬, 4 月卵巢重新往前发育。光唇鱼属短期分批产卵类型鱼类。 |
| 关键词: 光唇鱼 卵巢发育 卵母细胞 组织学 |
| DOI：10.11693/hyhz201302013013 |
| 分类号: |
| 基金项目:国家星火计划项目, 2011GA701001 号; 宁波市海洋与渔业局项目, 甬农办2012-50号 |
附件 |
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| HISTOLOGICAL OBSERVATION ON THE OVARY DEVELOPMENT OF CULTIVATED ACROSSOCHEILUS FASCIATUS |
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JIANG Jian-Hu1,2, DAI Hai-Ping1,3, ZHU Jun-Quan1, ZHANG Yu-Ming4, LEI Shi-Yong1
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1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;2.Zhejiang Institute of Freshwater Fisheries;3.Fishery Administration and Technical Service Station of Yinzhou;4.Xinchang Bureau of Water Conservancy
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| Abstract: |
| Morphological and histological features of ovary development of Acrossocheilus fasciatus were studied using dissecting and tissue sectioning techniques. The results show that the ovary development in the first sexual cycle contained I—IV continuous stages. Four to five months after hatching (MAH), the ovary was at stage I and contained stage I oocytes. Six to 18 MAH, the ovary was at stage II, and contained stage II oocytes, during which unilaminar follicle membrane formed. Nineteen to 20 MAH, the ovary was at stage III, in which stage III oocytes were contained, the number of layers in follicle membrane changed from one to two, the zona radiate formed between the plasma membrane and follicle membrane. Furthermore, the vacuole and yolk granule appeared in the cytoplasm. Twenty-one to 22 MAH, the ovary was at stage IV, stage IV oocytes were contained, the oocyte cytoplasm was full of yolk granule. Twenty-three to 24 MAH, the ovary was at stage V, stage V oocytes were contained, and the follicle membrane at this stage began to desquamate. Twenty-five MAH, the ovary was at stage VI, empty follicle and oocytes at stage II and III could be found. The spawning season of this species was from June to July, the ovary changed back to stage II in September; in December it changed back to stage III, and over all the winter the ovary remained at this stage. In April, the ovary continued to develop and entered into stage IV. According the morphological and histological characteristics of ovary development, A. fasciatus should be classified into the group of short-term batch-releasing patterned fish. |
| Key words: Acrossocheilus fasciatus ovary development oocyte histology |
