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引用本文:刘 臻,谢帝芝,周 毅,赵 琼,王赏初,孙 浪,鲁双庆.活性菌肽豆蛋白对鲫(Carassius auratus) 促生长的作用效果及机理分析.海洋与湖沼,2013,44(2):377-382.
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活性菌肽豆蛋白对鲫(Carassius auratus) 促生长的作用效果及机理分析
刘 臻1,2, 谢帝芝1,2,3, 周 毅1,2, 赵 琼1,2, 王赏初1,2, 孙 浪1,2, 鲁双庆1,2
1.长沙学院生物工程与环境科学系;2.长沙学院生物技术与营养研究所;3.汕头大学海洋生物研究所
摘要:
采用不同饲料配方投喂, 研究了普通豆粕(对照组)、发酵豆粕和活性菌肽豆蛋白配合饲料对鲫(Carassius auratus)生长性状影响的研究。结果表明, 活性菌肽豆蛋白组的相对体重生长率高于对照组41.87% (P<0.01)和发酵豆粕组19.11%; 血清中的三碘甲状腺原氨酸(T3)、甲状腺素(T4)、胰岛素样生长因子-I(IGF-I)和生长激素(GH)含量活性菌肽豆蛋白组最高, 其次是发酵豆粕组, 而对照组最低; 实时定量PCR检测显示: IGFs(IGF-I 和IGF-II)基因的表达量在活性菌肽豆蛋白组中最高, 其次是发酵豆粕组, 对照组的表达量最低。GHR基因的表达量在活性菌肽豆蛋白组和发酵豆粕组间无显著性差异, 但明显高于对照组。因此, 活性菌肽豆蛋白可能通过调控鲫血清GH、IGF-I、T3和T4等激素水平, 及肝脏GHR和IGFs基因的表达, 进而影响鲫的生长。
关键词:  活性菌肽豆蛋白    促生长  机理
DOI:10.11693/hyhz201302018018
分类号:
基金项目:国家自然科学基金资助项目, 31001114 号, 31272675 号; 长沙市科技局重点项目, K1205038-21 号
附件
THE GROWTH-PROMOTING EFFECT AND MECHANISM ANALYSIS OF ACTIVE MICROBIAL PEPTIDE SOYBEAN ON CRUCIAN CARP CARASSIUS AURATUS
LIU Zhen1,2, XIE Di-Zhi1,2,3, ZHOU Yi1,2, ZHAO Qiong1,2, WANG Shang-Chu1,2, SUN Lang1,2, LU Shuang-Qing1,2
1.Department of Biotechnology&Environment Science;2.Institute of Biotechnology and Nutrition, Changsha University;3.Marine Biology Institute, Shantou University
Abstract:
Using different feeding formulas, the effects of common soybean, fermented soybean and active microbial peptide soybean meals on growth appearance of crucian carp Carassius auratus were studied. The results indicat that the relative body weight growth rate was higher in active microbial peptide soybean group (41.87%) than control and fermented soybean group (19.11% higher); the serum triiodothyronine (T3), thyroxine (T4), insulin like growth factor I (IGF-I) and growth hormone (GH) levels of active microbial peptide soybean group were the highest with following fermented soybean group and the lowest in control group. Realtime-PCR indicated that IGFs (IGF-I and IGF-II) gene expressions were the highest in active microbial peptide soybean group, lower in fermented soybean group and the lowest in control group; GHR showed no significant difference between fermented soybean group and active microbial peptide soybean group, but both two groups were higher than control group. Thus, active microbial peptide soybean may regulate GH, IGF-I, T3 and T4 in serum as well as GHR and IGFsgene expression in liver to affect growth in C. auratus.
Key words:  active microbial peptide soybean  crucian carp Carassius auratus  growth-promoting  mechanism
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