| 摘要: |
| 采用常规急性实验方法, 研究了不同干露胁迫条件对脊尾白虾(Exopalaemon carinicauda)成活率的影响。结果表明, 保持虾体湿润并用冰块降温的P组干露胁迫12h后成活率为75%, 显著高于其它各实验组(P<0.05)。采用实时荧光定量PCR方法研究了不同干露胁迫条件对脊尾白虾血细胞和肝胰腺组织中热休克蛋白70(HSP70)和ferritin基因表达的影响。干露胁迫能诱导脊尾白虾血细胞、肝胰腺HSP70基因的表达上调, 肝胰腺中的高表达时间相对血细胞中出现的较早; 干露胁迫能诱导湿润低温P组脊尾白虾血细胞和肝胰腺ferritin基因的表达上调, 并显著高于对照组(P<0.05), 而且各组织ferritin 基因表达的上调时间具有差异性, 血细胞最先上调。其余实验组脊尾白虾各组织ferritin基因表达均下调, 并显著低于对照组(P<0.05)。上述结果表明, 在脊尾白虾受干露胁迫的耐受范围内, HSP70 和ferritin基因发挥抗氧化功能。 |
| 关键词: 脊尾白虾 干露 成活率 HSP70 Ferritin |
| DOI：10.11693/hyhz201302023023 |
| 分类号: |
| 基金项目:国家高技术研究发展计划课题项目“主要养殖甲壳类良种培育”(2012AA10A409); 国家虾产业技术体系(CARS-47); 公益性行业(农业)科研专项(201103034); 农业科技成果转化资金项目(2010GB23260589)和中央级公益性科研院所基本科研业务费(20603022012021) |
附件 |
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| THE EFFECT OF DRY EXPOSURE ON HSP70 AND FERRITIN GENE EXPRESSION LEVEL IN EXOPALAEMON CARINICAUDA |
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WANG Qi1,2, LI Jian2, LI Ji-Tao2, PAN Lu-Qing1, YANG Ai-Guo3
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1.Ocean University of China;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;3.Qingdao Huiquan Ocean Science and Technology Development Company Limited
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| Abstract: |
| The conventional acute test method was applied to detect the effect of dry exposure on survival rates in Exopalaemon carinicauda of different groups. The results showed that survival rate of group P was 75% after dry exposure for 12h, which was significantly higher than that of other groups (P<0.05). Quantitative real-time PCR was applied to detect the expression profiles of heat shock protein (HSP70) and ferritin gene in haemocytes and hepatopancreas of E. carinicaudaafter challenged by different dry exposure stress conditions. The results showed that Dry exposure stress could induce HSP70 gene expression in haemocytes and hepatopancreas of E. carinicauda, and HSP70 gene expression up-regulating time in hepatopancreas was significantly earlier than that in haemocytes (P<0.05). Dry exposure stress could induce fetrritin gene expression of group P (wet and low temperature) in blood cells and hepatopancreas of E. carinicauda, which was significantly higher than that of control group, and fetrritin gene expression up-regulating time in blood cells was significantly earlier than that in hepatopancreas (P<0.05). The expression levels of ferritin gene in other groups were significantly down-regulated, significantly lower than that of control group P (P<0.05). These results suggested that HSP70 and ferritin gene play important roles under dry exposure stresses by serving as antioxidant in E. carinicauda. |
| Key words: Exopalaemon carinicauda dry exposure survival HSP70 ferritin |
