| 摘要: |
| 采用酶学分析的方法, 以淡水组为对照, 进行了盐度变化对花鳗鲡(Anguilla marmorata)[体质量(10.70±0.92)g]和太平洋双色鳗鲡(A. bicolor pacifica)幼鳗[体质量(12.11±0.79)g]鳃丝及肾脏Na+/K+-ATP酶活力影响的研究。结果表明, 经不同的盐度(5、10、18)处理, 各处理组的花鳗鲡和太平洋双色鳗鲡幼鳗鳃丝Na+/K+-ATP酶活力均呈现先降低后升高最后再降低并趋于稳定的趋势。前者24h时达到最低值, 48h时盐度10处理组上升至与对照组无显著差异(P>0.05), 而盐度18处理组显著高于对照组(P<0.05)。后者6h时达到最低值, 12h时盐度5和18处理组达到最高值, 24h时盐度10处理组达到最高值。盐度变化对花鳗鲡和太平洋双色鳗鲡幼鳗肾脏Na+/K+-ATP酶活力影响不明显。太平洋双色鳗鲡幼鳗鳃丝Na+/K+-ATP酶酶活提升速度及提升幅度均强于花鳗鲡幼鳗, 故认为在盐度范围0—18时, 太平洋双色鳗鲡幼鳗比花鳗鲡幼鳗对盐度变化具有更强的适应能力。 |
| 关键词: 盐度 花鳗鲡 太平洋双色鳗鲡 幼鳗 Na+/K+-ATP酶 |
| DOI：10.11693/hyhz201303041041 |
| 分类号: |
| 基金项目:国家农业部公益性行业(农业)科研专项, nyhyzx07-043-03 号; 福建省科技计划重点项目, 2009N0046 号; 福建省教育厅重点项目, JA09157 号; 福建省科学技术厅项目, 2009N2003-1 号 |
附件 |
|
| EFFECTS OF SALINITY ON Na+/K+-ATPase ACTIVITY IN GILL AND KIDNEY OF ANGUILLA MARMORATA AND A. BICOLOR PACIFICA ELVER |
|
LUO Ming-Zhong1,2,3, GUAN Rui-Zhang1,2, JIN Heng1,2
|
|
1.Jimei University;2.Engineering Research Centre ofEel Modern Technical Industry, Ministry of Education;3.Institute of Hydrobiology, Chinese Academy of Sciences
|
| Abstract: |
| Effects of salinity on Na+/K+-ATPase activity in gill and kidney of Anguilla marmorata [body weight (10.70± 0.92)g] and A. bicolor pacifica elver [body weight (12.11±0.79)g] were studied in enzymology, and that of freshwater served as the control. The results suggest that after be treated in different salinities of 5, 10, and 18, the Na+/K+-ATPase activity in gill of them decreased at the beginning, and then increased and reached maximum; finally it decreased again and stabilized. The Na+/K+-ATPase activity in gill of A. marmorata elver reached minimum in 24h in all treated groups. In 48h, the activity in salinity 10 increased and showed no significant difference from the control group (P>0.05), but in salinity 18, it was much higher than that in control group (P<0.05). The activity in gill Na+/K+-ATPase of A. bicolor pacifica elver in all treated groups reached the minimum in 6h. In 12h, in salinity 5 and 18, the activity increased and reached the maximum, but in salinity 10 it reached maximum in 24h. Effects of salinity on Na+/K+-ATPase activity in kidney of the two elver species were minor. The finding indicate that A. bicolor pacifica elver are more tolertive to salinity change (in range of 0—18) than elver of A. marmorata. |
| Key words: salinity Anguilla marmorata Anguilla bicolor pacifica elver, Na+/K+-ATPase |
