| 摘要: |
| 本文以脂肪酶ADM47601为目标，使用多种材料对其进行了固定化处理。实验表明，海藻酸钠固定化脂肪酶最佳条件为3%（w/v）海藻酸钠，1%CaCl2，每毫升海藻酸钠添加600U脂肪酶，最大固定化酶活力回收率为17.11%。海藻酸钠-明胶固定化脂肪酶最佳条件为1%海藻酸钠，1%明胶，0.5%CaCl2，0.25%戊二醛，每毫升海藻酸钠-明胶混合液添加600U脂肪酶，最大固定化酶活力回收率为54.45%。壳聚糖固定化脂肪酶最佳固定化条件为2%壳聚糖，10% NaOH，1%乙酸，0.25%戊二醛，每克壳聚糖微球添加840U脂肪酶，最大固定化酶活力回收率为87.06%。聚乙烯醇固定化脂肪酶最佳条件为7.5%聚乙烯醇，2%海藻酸钠，1.5%CaCl2，每毫升聚乙烯醇添加360U脂肪酶，最大固定化酶活力回收率为33.22%。对比四种不同固定化酶方法，脂肪酶活力回收率高度高低顺序为：壳聚糖吸附交联法>海藻酸钠明胶协同包埋法>聚乙烯醇-硼酸法>海藻酸钠包埋法。 |
| 关键词: 海藻酸钠 壳聚糖 聚乙烯醇 脂肪酶 固定化 |
| DOI： |
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| 基金项目:国家“十二五”“863”项目（No.2011AA090703）、国际科技合作与交流专项（No.2011DFB30250）、黄海水产研究所级基本科研业务费资助（No.20603022013016）、青岛市科技计划项目（12-1-4-12-（2）-jch）、中国水产科学研究院基本科研业务费资助（2013A1002） |
相关附件： 海洋脂肪酶ADM47601固定化方法的研究 附图.xls |
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| Research on the immobilization of lipase ADM47601 |
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duanxiaochen,sheng jun,xu jiakun,hao jianhua and sun mi
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Dalian Ocean University，Yellow Sea Fisheries Research Institute,Yellow Sea Fisheries Research Institute,Yellow Sea Fisheries Research Institute,Yellow Sea Fisheries Research Institute,Yellow Sea Fisheries Research Institute
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| Abstract: |
| Using sodium alginate, sodium alginate-gelatin, chitosan and polyvinyl alcohol as the material to immobilize the marine lipase ADM47601.The optimal conditions of sodium immobilization is 3% sodium alginate,1% CaCl2,add lipase for 600U/mL sodium alginate,the maximum immobilized lipase activity recovery rate was 17.11%. The optimal conditions of sodium alginate-gelatin immobilization is 1% alginate, 0.5% CaCl2, 0.25% glutaraldehyde, add
Lipase for 600U/mL alginate-gelatine mixture, the maximum immobilized lipase activity recovery rate was 54.45%.The optimal conditions of chitosan immobilization is 2% chitosan,10% NaOH,1% acetic acid,0.25% glutaraldehyde, add lipase for 840U/g chitosan microspheres, the maximum immobilized lipase activity recovery rate was 87.06%.The optimal conditions of polyvinyl alcohol immobilized is 7.5%polyvinyl alcohol,2% sodium alginate,1.5Cl2,add lipase for 360U/mL polyvinyl alcohol, the maximum immobilized lipase activity recovery rate was 33.22%.Comparing four different immobilized methods, lipase activity recovery rate are chitosan> sodium alginate-gelatin> polyvinyl alcohol> alginate. |
| Key words: Alginate,Chitosan,Polyvinyl alcohol,Marine lipase,Immobilize |