引用本文:	段晓琛,盛军,徐甲坤,郝建华,于建生,孙谧.海洋脂肪酶ADM47601固定化方法的研究.海洋与湖沼,2013,44(5):1311-1317.

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海洋脂肪酶ADM47601固定化方法的研究
段晓琛¹, 盛军², 徐甲坤², 郝建华², 于建生³, 孙谧²
1.大连海洋大学食品学院,中国水产科学研究院黄海水产研究所,青岛市海洋酶工程重点实验室,农业部海洋渔业可持续发展重点实验室;2.中国水产科学研究院黄海水产研究所,青岛市海洋酶工程重点实验室,农业部海洋渔业可持续发展重点实验室;3.青岛科技大学化工学院

摘要:

采用海藻酸钠、壳聚糖、聚乙烯醇和明胶等材料, 进行对脂肪酶ADM47601的固定化研究。结果表明, 使用壳聚糖固定化脂肪酶, 在最优条件为2% (W/V)壳聚糖, 10% NaOH, 1%乙酸, 0.25%戊二醛, 每克载体添加840U脂肪酶时, 最大固定化酶活力回收率为87.06%。使用海藻酸钠-明胶固定化脂肪, 在最优固定化条件下, 最大固定化酶活力回收率为54.45%。使用聚乙烯醇固定化脂肪酶, 在最优固定化条件下, 最大固定化酶活力回收率为33.22%。使用海藻酸钠固定化脂肪酶, 在最优固定化条件下, 最大固定化酶活力回收率为17.11%。对比四种不同固定化酶方法, 脂肪酶活力回收率高度高低顺序为: 壳聚糖吸附交联法>海藻酸钠明胶协同包埋法>聚乙烯醇-硼酸法>海藻酸钠包埋法。

关键词: 海藻酸钠壳聚糖聚乙烯醇海洋脂肪酶固定化

DOI：10.11693/hyhz201305028028

分类号:

基金项目:国家“863”项目, 2011AA090703号; 国际科技合作与交流专项, 2011DFB30250号; 中国水产科学研究院黄海水产研究所所级基本科研业务费资助, 20603022013016号; 青岛市科技计划项目, 12-1-4-12-(2)-jch号; 中国水产科学研究院基本科研业务费资助, 2013A1002号。

附件

RESEARCH ON THE IMMOBILIZATION OF LIPASE ADM47601

DUAN Xiao-Chen¹, SHENG Jun², XU Jia-Kun², HAO Jian-Hua², YU Jian-Sheng³, SUN Mi²

1.College of Food Science and Engineering, Dalian Ocean University,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesQingdao Key Laboratory of Marine Enzyme Engineering Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture ;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesQingdao Key Laboratory of Marine Enzyme Engineering Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture ;3.College of Chemical Engineering, Qingdao University of Science and Technology

Abstract:

We treated marine lipase ADM47601 with sodium alginate, mixture of sodium alginate and gelatin, chitosan, and polyvinyl alcohol to study the immobilization properties of the lipase. The results are: (1) immobilization with sodium alginate, at the optimal conditions of 3% sodium alginate, 1% CaCl2, 600U/mL lipase addition, the maximum immobilized lipase activity recovery rate was 17.11%; (2) with the mixture of sodium alginate and gelatin, at 1% alginate, 0.5% CaCl2, 0.25% glutaraldehyde, 600U/mL lipase addition, the maximum recovery rate was 54.45%; (3) with chitosan, at 2% chitosan, 10% NaOH, 1% acetic acid, 0.25% glutaraldehyde, 840U/g lipase addition, the maximum rate could reach 87.06%; and (4) with polyvinyl alcohol, at conditions of 7.5% polyvinyl alcohol, 2% sodium alginate, 1.5% CaCl2, and 360U/mL lipase addition, the maximum rate was 33.22%. Therefore, among the four materials, chitosan is the best for the highest recovery, followed by sodium-alginate-gelatin mixture, polyvinyl alcohol, and sodium alginate.

Key words: alginate chitosan polyvinyl alcohol marine lipase immobilize