| 摘要: |
| 采用胸腔注射植物血球凝集素(phytohemagglutinin, PHA)及秋水仙素溶液, 取活体头肾组织低渗、固定和空气干燥, 分析比较了(Terapon theraps)、细鳞(Terapon jarbua,/i>)、尖吻 (Rhynchopelates oxyrhynchus)、叉牙 (Helotes sexlineatus)、列牙 (Pelates quadrilineatus)和高体革 (Scortum barcoo)的核型。结果表明, 6种鱼中期染色体均为二倍体, 除尖吻外均未发现异型性染色体、随体和次缢痕。其核型如下: 的核型为2n=48=48t, 臂数: NF=48; 细鳞的核型为2n=48=48t, NF=48; 叉牙2n=48=48t, NF=48; 列牙的核型为2n=48=48t, NF=48; 高体革2n=48=2m+2sm+44t, NF=52; 尖吻 2n=48=4m+2st+42t, NF=52。科这6种鱼染色体均属于典型的高位类群。同时, 将科6种鱼与前人已完成的科其它鱼类的核型进行比较, 发现尖吻的核型出现异型性染色体, 并对尖吻出现罕见的异型性染色体进行分析讨论。除高体革外, 其余5种核型为国内首次报道。 |
| 关键词: 科 染色体 核型 异型性染色体 |
| DOI：10.11693/hyhz201305037037 |
| 分类号: |
| 基金项目:国家公益性行业(农业)重大专项, 201303048号; 广东省科技专项资金社会主义新农村建设项目, 2012A020602063号; 广东省科技兴海重点项目, 粤财农2011587号。 |
附件 |
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| KARYOTYPES OF SIX TERAPONIDAE FISH SPECIES |
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SHU Hu1, HUANG Cui-Ying1, LIU Li2, HOU Li-Ping1, ZHANG Hai-Fa3, ZHAO Hui-Hong2
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1.School of Life Science, Guangzhou University;2. Animal Science College, South China Agricultural University;3.Guangdong Daya Bay Fisheries Development Center
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| Abstract: |
| The karyotypes of 6 species of Teraponidae fishes were analyzed. The PHA and colchicine were injected in vivo, and kidney cells were collected and treated in lower osmotic pressure and air-drying technique. The result show that six species had diploid chromosome style, and satellite chromosome; the secondary constrictions and sex chromosome were not found except for Rhynchopelates oxyrhynchus. The karyotypes were formulated as: 2n=48=48t, NF=48 for Terapon theraps, Helotes sexlineatus, and Pelates quadrilineatus; 2n=48=4m+44t, NF=52 for Scortum barcoo, and 2n=48=4m+2st+42t, NF=52 for R. oxyrhynchus. Abnormal sex chromosomes appeared in the karyotypes of R. oxyrhynchus. The karyotypes of the six Teraponids are typical of high position in phylogeny. By comparing the karyotypes of other species in Order Teraponidae, we find that karyotype of R. oxyrhynchus is very different from those of T. jarbua and P. quadrilineatus, which should be studied in the future. Except for S. barcoo, the karyotypes of the other 5 species were reported for the first time in China. |
| Key words: Teraponidae chromosome karyotype abnormal sex chromosomes |
