| 摘要: |
| 以罗非鱼(Tilapia zillii)鱼鳞为原料, 考察提取pH和温度对明胶蛋白组分及其性质的影响。结果表明, 鱼鳞明胶蛋白在浸提pH大于5时容易发生酶解, pH小于3时会发生热降解, 而在pH 4下酶解和热降解均被有效抑制。在pH 4下利用40℃浸提明胶时, 蛋白的提取率只有1.84%, 提取的明胶中亚氨基酸比例低而钙、磷、钠、镁等矿物质含量高, 因而无法形成凝胶和蛋白膜。当浸提温度提高到50℃及以上时, 明胶提取率大幅增加, 明胶中亚氨基酸比例上升且矿物质含量比例下降。另外, 在pH 4、70℃下浸提的明胶其凝胶强度可达283g, 可形成机械强度为50MPa的蛋白膜, 表明该条件提取的鱼鳞明胶具有替代哺乳动物明胶的潜力。 |
| 关键词: 鱼鳞明胶 pH 温度 凝胶性能 成膜性能 |
| DOI：10.11693/hyhz201305041041 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 31271984号; 福建省高校新世纪优秀人才支持计划, JA11143号。 |
附件 |
|
| EFFECT OF pH AND TEMPERATURE ON GELATIN EXTRACTION FROM TILAPIA (TILAPIA ZILLII) SCALES |
|
ZHENG Hui-Bin, WENG Wu-Yin, SU Wen-Jin, HAO Geng-Xin
|
|
College of Bioengineering, Jimei University
|
| Abstract: |
| The effects of pH and temperature on extraction of gelatin from tilapia (Tilapia zillii) scales were investigated. The results show that at pH>5, enzymatic degradation of gelatin took place, while at pH<3, acidic thermal hydrolysis would occur. At pH 4, both the enzymatic degradation and thermal acid hydrolysis could be prevented regardless of temperature. At 40℃, the gelatin yield was low (1.84%), along with low content of imino acid, high content of mineral components such as calcium, phosphorus, sodium and magnesium, which prevented gel or film from formation. At 50℃, the protein yield and imino acid content substantially increased, and mineral content decreased too. Texture profile analysis revealed optimal conditions for producing best gelatin: pH 4 at 70℃, under which the gel strength was 283g and film tensile strength was 50MPa, providing potential substitution for mammalian gelatin. |
| Key words: scale gelatin pH temperature gelling properties film-forming properties |
