引用本文:	张群英,黄鹤忠,袁文成,李文龙,雍福和,徐雪英,张平.中华鳖(Pelodiscus sinensis)EST-SSR标记与生长性状相关性分析.海洋与湖沼,2013,44(6):1654-1660.

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中华鳖(Pelodiscus sinensis)EST-SSR标记与生长性状相关性分析
张群英¹, 黄鹤忠¹, 袁文成¹, 李文龙¹, 雍福和², 徐雪英³, 张平⁴
1.苏州大学基础医学与生物科学学院;2.江苏省苏州市吴中区胥口镇马舍特种水产养殖场;3.江苏省苏州市水产技术推广站;4.江苏省徐州市睢宁县水产技术指导站

摘要:

采用中华鳖转录组测序方法开发了一系列EST-SSR引物, 对60 只中华鳖(Pelodiscus sinensis)的遗传多样性和标记-性状相关性进行研究, 结果表明, 15 对EST-SSR引物扩增结果达到了高度多态水平, 平均的有效等位基因数、期望杂合度和多态性信息含量分别为2.7633、0.5961 和0.539177。C8387位点分别与体长、体重、背甲长和裙边宽显著相关(P<0.05)和极显著相关(P<0.01), CC型、AB型分别为4种性状的优势、劣势基因型; C211位点分别与体重、背甲长和裙边宽呈极显著相关(P<0.01)和显著相关(P<0.05), 其中等位基因C与体重呈负相关, CC 型是裙边宽、背甲长性状的劣势基因型, 而BB型和AB型分别是裙边宽和体重的优势基因型; C5670位点与体重和背甲长分别呈极显著相关(P<0.01)和显著相关(P<0.05), 其中BB型和AA型分别是体重和背甲长的优势、劣势基因型; C1312 和C13038位点均与体重、背甲长极显著相关(P<0.01), 且C1312上的BC型、C13038上的BB型分别是中华鳖体重、背甲长性状的优势基因型。

关键词: 中华鳖 EST-SSR标记生长性状相关性分析

DOI：10.11693/hyhz201306036

分类号:

基金项目:江苏省科技支撑项目, BE2012357号; 江苏省睢宁县水产原良种体系建设项目, PJ2012-10号。

附件

CORRELATION OF EST-SSR MARKERS WITH GROWTH TRAITS IN CHINESE SOFT-SHELLED TURTLES (PELODISCUS SINENSIS)

ZHANG Qun-Ying¹, HUANG He-Zhong¹, YUAN Wen-Cheng¹, LI Wen-Long¹, YONG Fu-He², XU Xue-Ying³, ZHANG Ping⁴

1.School of Medicine and Life Sciences, Medical College of Soochow University, Fisheries Research Institute of Soochow University;2.Mashe Special Aquaculture Farms in Xukou Town, Wuzhong District, Suzhou City, Jiangsu Province;3.Fisheries Technology Extension Station of Suzhou City, Jiangsu Province;4.Fisheries Technology Guide Station of Suining County in Xuzhou City, Jiangsu Province

Abstract:

15 polymorphic microsatellites were developed from the transcriptome sequencing EST database of Chinese soft-shelled turtles (Pelodiscus sinensis) to analyze the genetic diversity and linkage of 60 individuals. The result indicate that: 15 EST-SSR markers obtained amplified products successfully in a high polymorphism level; the number of mean valid alleles was 2.7633; the value of mean expected heterozygosity was 0.5961; and the mean polymorphism information content (PIC) was 0.539177. The linkage analysis showed the locus C8387 had a significant impact on body weight, body length, carapace length and a extremely significant difference in side width (P<0.01), and CC at C8387 was a favorable genotype for the four growth traits while AB was a disadvantage genotype; locus C211 showed an extremely significant impact on body weight, carapace length (P<0.01) and a significant impact on the side width (P<0.05), among them allele C was negatively associated with the body weight and genotype CC was disadvantage genotype of carapace length and side width, while BB and AB were favorable genotypes of side width and body weight respectively; locus C5670 had an extremely significant impact on body weight (P<0.01) and a significant impact on carapace length (P<0.05), in which BB was the dominant genotype of body weight and carapace length while the AA was the negative genotype; the locus C1312 and C13038 had a extremely significant correlation of body weight, carapace length, respectively (P<0.01), in which BC of locus C1312 and BB of C13038 was dominant genotype of body weight and carapace length, respectively.

Key words: Pelodiscus sinensis EST-SSR marker growth trait correlation analysis