| 摘要: |
| 为了研究全浸15天不锈钢表面生物膜的形成情况, 首先采用SEM及荧光显微镜进行观察, 显示全浸15天不锈钢表面已有大量微生物及其腐蚀产物附着, 表明其表面生物膜已经形成; 进一步通过EDS对不锈钢表面进行元素分析, 发现实海全浸15天的316L不锈钢表面出现了大量组成生物体的C、P等基本元素, 并且还出现了不锈钢基体中所不存在而存在于生物膜及其代谢产物中O等元素, 进一步证明了不锈钢表面生物膜的形成。采用16S rRNA基因结合PCR-RFLP技术分析手段对实海全浸15天的316L不锈钢表面生物膜细菌群落的多样性进行了研究, 发现316L不锈钢表面附着细菌的OTU数为24, 分别属于变形菌门和拟杆菌门, 其中α-变形菌的OTU数是18, 占总克隆数的72.3%, 为绝对优势种群。 |
| 关键词: 生物膜 316L不锈钢 细菌多样性 PCR-RFLP 生物腐蚀 |
| DOI：10.11693/hyhz20130700105 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 40976046 号; 国家重点基础研究发展计划(973 计划), 2014CB643304 号 |
附件 |
|
| DIVERSITY OF BACTERIA COMMUNITY OF BIOFILM ON STAINLESS STEEL SURFACE IMMERSED IN SEAWATER |
|
chenyongwei and duanjizhou
|
|
College of Marine Life Sciences,Ocean university of China,Institute of Oceanology,Chinese academy of Sciences
|
| Abstract: |
| We studied the microbial community and its metabolites of the biofilm on 316L stainless steel after 15d- immersion in seawater by scanning electronic microscope and fluorescence microscope. The EDS (energy dispersive spectro?scopy) shows that on the stainless steel surface, it generated a large number of elements C and P, and other basic elements of organisms, and some new elements, such as oxygen, indicating evidence of biofilmformation. We applied 16S rRNA gene clone library technique and PCR-RFLP analysis to generate a comprehensive view of the diversities and compositions of the biofilms. The results show that the number of bacteria OTUs on 316L stainless steel is 24, belonging to Proteobacteria and Bacteroidetes. Among them, the OTU numberof α- proteobacteria is 18, accounting for 72.3% of the total number of clones, as a dominant population. |
| Key words: biofilm 316L stainless steel bacterial diversity PCR-RFLP microbiologically influenced corrosion |
