| 引用本文: | 韩龙江,刘清华,纪利芹,温海深,黄 雯,张国范,李 军.太平洋牡蛎(Crassostrea gigas)担轮幼虫的超低温保存研究.海洋与湖沼,2014,45(6):1258-1263. |
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| 太平洋牡蛎(Crassostrea gigas)担轮幼虫的超低温保存研究 |
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韩龙江,刘清华,纪利芹,温海深,黄雯,张国范,李军
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中国海洋大学 水产学院 青岛,中国科学院 海洋研究所 青岛,海大水产学院,中国海洋大学 水产学院,中国科学院大学;中国科学院 海洋研究所 青岛;,中国科学院 海洋研究所 青岛,中国科学院 海洋研究所 青岛
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| 摘要: |
| 本文研究了六种抗冻保护剂GLY(甘油)、DMSO(二甲基亚砜)、PG(丙二醇)、EG(乙二醇)、DMA(二甲基乙酰胺)和MeOH(甲醇)在三种不同浓度下(5%、10%、15%, v/v)对太平洋牡蛎(Crassostrea gigas)担轮幼虫的毒性作用及冷冻保存的影响。太平洋牡蛎担轮幼虫毒性实验证明: 抗冻保护剂的种类和浓度对太平洋牡蛎担轮幼虫产生的毒性强弱不同, 在抗冻保护剂浓度为5%时, GLY、DMSO、PG、EG对太平洋牡蛎的毒性作用显著低于其他抗冻保护剂(P <0.05); 随着抗冻保护剂浓度增加到10%, GLY、DMSO、EG、MeOH对太平洋牡蛎担轮幼虫的毒性作用显著低于其他抗冻保护剂(P <0.05); 而抗冻保护剂浓度为15%时, GLY、DMSO对太平洋牡蛎担轮幼虫的毒性作用显著低于其他抗冻保护剂(P <0.05), 且随着抗冻保护剂浓度的升高, 其对太平洋牡蛎担轮幼虫的毒性作用也增大。太平洋牡蛎担轮幼虫的超低温冷冻保存实验证明: 以10% DMSO作为抗冻保护剂, 采用分布降温法冷冻保存太平洋牡蛎担轮幼虫, 28°C水浴解冻后担轮幼虫运动率达到(73.00±2.00)%, 显著高于其他实验组(P <0.05), 其次为GLY组, 且各浓度间差异不显著(P >0.05)。该研究为太平洋牡蛎及其他贝类胚胎保存提供了科学依据, 在贝类遗传育种和生物技术等方面具有一定的应用前景, 具有重要的生产实践价值。 |
| 关键词: 太平洋牡蛎 担轮幼虫 抗冻保护剂 毒性 冷冻保存 |
| DOI：10.11693/hyhz20131100187 |
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| 基金项目:国家高技术研究发展计划(863 计划)项目, 2012AA10A402 号; 海洋药源生物种质资源库建设, 12PYY001SF08 号; 国家重点基础研究发展计划(973 计划)项目, 2010CB126401 号; 水产种质资源平台运行服务 |
相关附件： 附件 海湖投稿.zip |
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| CRYOPRESERVATION ON TROCHOPHORE LARVAE OF CRASSOSTREA GIGAS |
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han longjiang,liuqinghua,jiliqin,wenhaishen,huangwen,zhangguofan and lijun
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Fisheries College, Ocean University of China,iocas,Fisheries College, Ocean University of China,Fisheries College, Ocean University of China,iocas,iocas,iocas
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| Abstract: |
| We studied six antifreeze protectants (GLY (glycerine), DMSO (dimethyl sulfoxide), PG (propylene glycol), EG (ethylene glycol), DMA (dimethyl acetamide) and MeOH (methanol)) at three concentrations (5%, 10%, and 15%, v/v) to test the toxic impact on the cryopreservation of trochophore larvae of the oyster. Result shows that the type and the concentration have different impacts on the larvae. At 5% concentration, GLY, DMSO, PG, and EG showed significantly lower toxicity than other antifreeze’s (P <0.05). At 10%, the toxicity of GLY, DMSO, EG, and MeOH remained significantly lower than other ones’ (P <0.05). Increasing to 15%, GLY and DMSO had still significantly lower toxicity among all (P <0.05). In general, increasing the concentration of antifreeze protectants would increase the toxic effect. In addition, the experiment showed that stepwise cooling with 10% DMSO as the antifreeze protectants was a best protocol to cryopreserve the trochophore larvae, and the motility of the trochophore larvae thawed in water bath at 28°C reached 73.00%±2.00%, which is significantly higher than other groups’ (P <0.05). GLY was the second best choice and no significant difference was shown among its concentration groups (P >0.05). This research provided a scientific basis for the cryopreservation of embryo for oysters and other shellfish, which is important for the production. |
| Key words: the Pacific oyster trochophore larvae antifreeze protectant toxicity cryopreservation |
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