| 引用本文: | 胡 娇,张 飞,徐晓津,苏永全,覃映雪,马 英,张 艺,韩坤煌,鄢庆枇.病原分离鉴定及致病性研究.海洋与湖沼,2014,45(2):409-417. |
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| 病原分离鉴定及致病性研究 |
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胡娇,张飞,徐晓津,苏永全,覃映雪,马英,张艺,韩坤煌,鄢庆枇
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集美大学 水产学院 农业部东海海水健康养殖重点实验室,集美大学 水产学院 农业部东海海水健康养殖重点实验室,集美大学 水产学院 农业部东海海水健康养殖重点实验室,厦门大学 海洋与地球学院,集美大学 水产学院 农业部东海海水健康养殖重点实验室,集美大学 水产学院 农业部东海海水健康养殖重点实验室,宁德市水产技术推广站;宁德市水产技术推广站,宁德市富发水产有限公司,集美大学 水产学院 农业部东海海水健康养殖重点实验室
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| 摘要: |
| 于2013年4月从宁德患内脏白点病大黄鱼(Pseudosciaena crocea)中分离得到两株优势菌NZBD9和NZBD11, 这两株菌在16—19°C条件下回归感染能引起大黄鱼内脏白点病, 而在7—10°C和24—27°C条件下同样的人工感染不能致病, 从而证实这两株菌为大黄鱼内脏白点病的病原菌。经16S rDNA基因的测序和时间飞行质谱微生物鉴定仪分析, NZBD9 和NZBD11同为变形假单胞菌。药敏性实验结果显示NZBD9对庆大霉素、诺氟沙星和四环素等7种药物高度敏感。组织病理学观察结果显示病鱼的肝脏、脾脏、头肾等组织中均出现明显病症, 如变性和坏死。 |
| 关键词: 大黄鱼 变形假单胞菌 内脏白点病 致病性 |
| DOI：10.11693/hyhz20140300078 |
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| 基金项目:公益性行业(农业)科研专项, 200903029 号; 国家自然科学基金项目, 31272699 号, 41176115 号。 |
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| ISOLATION, IDENTIFICATION AND VIRULENCE OF THE PATHOGEN OF WHITE-SPOTS DISEASE IN INTERNAL ORGANS OF PSEUDOSCIAENA CROCEA |
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hu jiao,zhang fei,xu xiao jin,su yong quan,qin ying xue,ma ying,zhang yi,han kun huang and YAN Qing-Pi
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Fisheries College of Jimei University,Fisheries College of Jimei University,Fisheries College of Jimei University,College of Ocean and Earth Sciences, Xiamen University,,Fisheries College of Jimei University,Fisheries College of Jimei University,Ningde Aquatic Product Technology Promotion Department,Ningde Fufa Aquatic Product Co. Ltd,Fisheries College of Jimei University
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| Abstract: |
| Two dominant strains (NZBD9 and NZBD11) were isolated from internal organs of Pseudosciaena crocea suffered from white-spots disease in April 2013. Both strains could cause the disease artificially in the internal organs in 16—19℃but at 7—10℃and 24—27℃. The results indicate that the two strains were the causative agent of the disease in farmed P. c ro c e a. Both were identified as Pseudomonas plecoglossicidaby time-of-flight mass spectrometer in 16S rDNA sequences. Experiments in susceptibility showed that NZBD9 is highly sensitive to 7 drugs including gentamicin, norfloxacin, and tetracycline. Histopathological observation showed that affected fish had clear symptoms of degeneration and necrosis in liver, kidney and spleen. |
| Key words: Pseudosciaena crocea Pseudomonas plecoglossicida pathogenicity internal organs white-spots disease |