| 摘要: |
| 以1月龄棘胸蛙蝌蚪[体长(1.694±0.121)cm,体重(0.548±0.062)g]为实验对象,在观察水温对蝌蚪行为和尾部皮肤表观色泽Hunter L、A、B值影响的基础上,设置19℃(对照组)、21.5℃、23℃、24.5℃、26℃五个水温梯度,并以96h为水温胁迫时长,12d为水温恢复时长,研究了水温胁迫及恢复条件下棘胸蛙蝌蚪尾部皮肤ATP酶和抗氧化酶(SOD和CAT),以及肝脏抗氧化酶(SOD,CAT,GSH)活力的变化特征。结果表明:(1)棘胸蛙蝌蚪分布水层、集群状况、个体移动率均与其培育水温关系密切,14℃和26℃分别为可显著改变蝌蚪分布水层和个体移动率的水温临界; (2) L值可作为表征蝌蚪尾部皮肤感应培育水温的指示色差,水温26℃为致其发生显著改变的高温临界; (3)蝌蚪尾部皮肤ATP酶耐受水温的最适高限为24.5℃,适宜范围为21.5-24.5℃; (4)蝌蚪尾部皮肤和肝脏表露SOD酶活力的最适水温均为23℃,而表露CAT酶活力的最适水温则分别为23℃和24.5℃,水温26℃时肝脏SOD酶已表露受损; (5)蝌蚪肝脏表露GSH酶活力的最适水温为24.5℃,水温26℃为该酶表露受损的高温临界。 |
| 关键词: 水温 棘胸蛙蝌蚪 行为 色差 尾部 肝脏 ATP 酶 抗氧化酶 |
| DOI：10.11693/hyhz20141100301 |
| 分类号: |
| 基金项目:余姚市与浙江海洋学院市院合作项目:棘胸蛙集约化高效养殖关键技术集成与综合示范,2013-2016;宁波市鄞州区科技攻关项目:棘胸蛙驯养与繁育技术研究与示范,2013-2014。 |
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| IMPACT OF WATER TEMPERATURE ON PAA SPINOSA TADPOLE BEHAVIOR AND ENZYME ACTIVITY IN TAIL SKIN AND LIVER |
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zhuweidong,rensuyi,shentu yan,zoulichang and wangzhizheng
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Zhejiang Ocean University,Zhejiang Ocean University,Zhejiang Ocean University,Zhejiang Ocean University,Zhejiang Ocean University
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| Abstract: |
| We studied water temperature influence on Paa spinosa tadpoles. One-month tadpoles [body length (1.694±0.121)cm, body weight (0.548±0.062)g] were sampled and tested under 19 (control group), 21.5, 23, 24.5 and 26℃, under which the tadpole behavior was monitored and tail skin appearance in chromatic aberration observed in Hunter L, A, B color value. We studied the variations in the activities of ATP enzyme, antioxidant enzyme on tail skin, and antioxidant enzyme on liver and set time lengths for temperature stress at 96h and recovery at 12d. The results show that water temperature affected significantly the water zonation, and animal flocking and individual movement. The critical water temperatures in which water zonation formed and individual movement significantly changed were 14℃ and 26℃. The L value could be used to indicate color change on the tail skin; and it remarkably changed at 26℃, the critical high temperature. The tolerable temperature for ATP enzyme activity on tail skin ranged 21.5-24.5℃. The optimum water temperature for SOD enzyme activity in tail skin and liver was 23℃, while 23℃ and 24.5℃ for CAT enzyme activity. In addition, when water temperature reached 26℃, SOD enzyme activity in liver decreased with obvious damage in the liver. The optimum water temperature for GSH enzyme activity in liver was 24.5℃, and the critical temperature was 26℃. |
| Key words: water temperature Paa spinosa tadpole behavior chromatic aberration tail liver ATP enzyme antioxidant enzyme |
