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引用本文:郭建丽,田岳强,马爱军,黄智慧,商晓梅,王新安,杨志,曲江波.大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)性状的微卫星分子标记研究.海洋与湖沼,2015,46(1):157-164.
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大菱鲆(Scophthalmus maximus)抗鳗弧菌(Vibrio anguillarum)性状的微卫星分子标记研究
郭建丽,田岳强,马爱军,黄智慧,商晓梅,王新安,杨志,曲江波
1.中国水产科学研究院黄海水产研究所 农业部海洋渔业可持续发展重点实验室 青岛市海水鱼类种子工程与生物技术重点实验室 青岛 266071;2.上海海洋大学 上海 201306;3.烟台开发区天源水产有限公司 烟台 264003
摘要:
采用微卫星分子标记技术分析验证了133 对微卫星分子标记, 以期为大菱鲆(Scophthalmus maximus)育种提供合适的抗鳗弧菌分子标记。实验鱼经鳗弧菌(Vibrio anguillarum)感染, 分为非抗病组与抗病组。对两组鱼PCR 扩增出的差异条带进行个体统计, 再进行微卫星位点与抗鳗弧菌的相关性分析, 并分析对比了两组鱼的遗传多样性。结果表明, 微卫星位点Sma-USC108、Sma-USC141 的等位基因片段分别在215bp 和200bp 与抗鳗弧菌性状的正相关性极显著, 相关系数分别达到0.363和0.407(P<0.01); 微卫星位点FF0911、Sma-USC62、Sma-USC279 的等位基因片段分别在189bp、163bp 和236bp 与抗鳗弧菌性状的负相关性极显著, 相关系数分别达到-0.377、-0.364 和-0.363(P<0.01)。经过二次验证, 最终确定Sma-USC141 和FF0911 两个微卫星位点可指导大菱鲆抗鳗弧菌的辅助育种。两组大菱鲆的群体遗传多样性分析对比表明: 抗病群体与非抗病群体的遗传变异水平较低, 两个群体的遗传多样性水平相当。
关键词:  大菱鲆  微卫星标记  鳗弧菌  遗传多样性
DOI:10.11693/hyhz20140700196
分类号:
基金项目:现代农业产业技术体系建设专项资金资助, CARS-50-G01号。
附件
THE SCREENING OF MICROSATELLITES MOLECULAR MARKERS WITH REFERENCE TO ANTI-VIBRIO ANGUILLARUM OF TURBOT SCOPHTHALMUS MAXIMUS
GUO Jian-Li1,2,3, TIAN Yue-Qiang1,2,3,4, MA Ai-Jun1,2,3, HUANG Zhi-Hui1,2,3, SHANG Xiao-Mei1,2,3, WANG Xin-An1,2,3, YANG Zhi5, QU Jiang-Bo5
1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology;3.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P. R. China, Qingdao 266071, China;4.Shanghai Ocean University, Shanghai 201306, China;5.Yantai Tianyuan Aquatic Limited Corporation, Yantai 264003, China
Abstract:
We explored 133 pairs of microsatellite markers included in GenBank to obtain suitable molecular markers that closely associated with anti-Vibrio anguillarum for turbot (Scophthalmus maximus L.) breeding. The experimental turbot by intraperitoneal injection with Vibrio anguillarum were divided into two groups: susceptible group and resistant group. The differential bands in the two turbot groups were selected, from which the correlation between the upper anti-Vibrio anguillarum and the microsatellite loci were analyzed, and the genetic diversity compared. The results show that alleles of Sma-USC108 and Sma-USC141 at 215bp and 200bp respectively were extremely significantly correlated with anti-Vibrio anguillarum with the Pearson correlation value of 0.363 and 0.407. On the other hand, alleles of FF0911, Sma-USC62, and Sma-USC279 at 189bp, 163bp, and 236bp, respectively, were extremely significantly negative-correlated with anti-Vibrio anguillarum, with the Pearson correlation values at -0.377, -0.364, and -0.363 (P<0.01), respectively. We verified the above result with a second trial and determined that Sma-USC141 and FF0911 is useful for further molecular assistant selection of anti-Vibrio anguillarum turbot. Comparative analysis showed that they two groups were in low genetic variation at a same-level of genetic diversity.
Key words:  Scophthalmus maximus L.  microsatellites molecular markers  Vibrio anguillarum  genetic diversity
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