| 摘要: |
| 以不同浓度的氯化钾(KCl)溶液处理仿刺参(Apostichopus japonicus)樽形幼虫, 处理不同时间后, 分析KCl 对仿刺参幼虫附着变态的诱导作用。结果表明, 使用较低浓度的KCl 溶液(10、30和50 mmol/L)分别诱导仿刺参樽形幼虫6、12 和24 h 均可以显著提高其附着变态率, 诱导24 h 附着变态率分别提高27.67%、32.67%和19.67%。较高浓度的KCl 溶液(100、300 和500 mmol/L)也可以诱导仿刺参幼虫附着变态, 但随着KCl 浓度的升高和诱导时间的延长, 幼虫死亡率逐渐升高。低浓度KCl 溶液, 特别是在30 mmol/L 诱导仿刺参幼虫12 h 时, 可以显著提高幼虫的附着变态率, 增加发育的同步性, 降低死亡率, 对仿刺参幼虫变态的诱导效果较好。 |
| 关键词: KCl 仿刺参 樽形幼虫 变态 诱导 |
| DOI：10.11693/hyhz20140900239 |
| 分类号: |
| 基金项目:海洋公益性行业科研专项经费项目,201305001号;山东省优秀中青年科学家科研奖励基金,BS2012HZ027号;烟台市科技发展计划项目,2012133号;山东省现代农业产业技术体系——刺参创新团队(SDAIT-08);水生动物营养与饲料“泰山学者”岗位经费资助。 |
附件 |
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| SETTLEMENT AND METAMORPHOSIS OF SEA CUCUMBER APOSTICHOPUS JAPONICUS LARVAE INDUCED BY POTASSIUM CHLORIDE |
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WEI Xiu-Mei1, YANG Ding-Long1, WANG Sheng1, JIANG Xu1, LIU Xiang-Quan1, YANG Jian-Min1, FANG Jing-Hui2
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1.Shandong Provincial Key Laboratory of Marine Ecology Restoration, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;2.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Qingdao 266071, China
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| Abstract: |
| The impact of potassium chloride (KCl) solutions on settlement and metamorphosis of doliolaria larvae of sea cucumber (Apostichopus japonicus) was studied in present work. Exposure of KCl was set in a spectrum of concentration at 10, 30 and 50 mmol/L for 6, 12, and 24 h. The results showed that the metamorphosis rates were significantly increased in KCl exposure at 10, 30, and 50 mmol/L for 6, 12, and 24 h; for example in 24-h group, it increased by 27.67%, 32.67%, and 19.67%, respectively. Although higher concentrations (100, 300, and 500 mmol/L) could also induce the metamorphosis, the mortality rate might rise with increasing KCl concentration and induction time. Overall, 12 h induction in low concentrations of KCl solution, e.g., 30 mmol/L, could significantly enhance the metamorphosis rates. Therefore, the combination of KCl treatment for doliolaria larvae in 30 mmol/L for 12 h was recommended in favor of the synchronism development in less mortality rate for better induction effect. |
| Key words: KCl sea cucumber doliolarias metamorphosis induction |
