| 摘要: |
| 本研究采用高通量测序技术进行了翘嘴鳜(Siniperca chuatsi)转录组学研究, 并扩增分析了EST-SSR 分子标记的多态性, 以期保护翘嘴鳜种质资源及良种选育, 发掘其功能性SSR 分子标记。结果表明, 在翘嘴鳜转录组测序得到的51245 条unigenes 中共检测出14094 个EST-SSR 位点, 分布于35285 条Unigenes 中, 出现频率为27.51%。翘嘴鳜转录组EST-SSR 位点的序列总长度达到195086bp, EST-SSR 位点长度分布从10—25 个碱基不等, 平均长度13bp; 翘嘴鳜转录组EST-SSR 位点共有90 种重复基元; 其中, 二核苷酸和三核苷酸重复单元是主导类型, 分别占总SSR 的30.62%和14.23%, 且GT/AC、AAG/CCT 分别占总SSR 的32.12%和6.36%。随机选取100 对经济性状相关EST-SSR 引物的扩增产物中有24.7%表现为多态性, 可作为功能性SSR 分子标记开发。 |
| 关键词: 翘嘴鳜 转录组 EST-SSR 信息 多态性 |
| DOI：10.11693/hyhz20140900248 |
| 分类号: |
| 基金项目:江苏省水产三新工程项目,Y2014-37号;江苏省区域现代农业与环境保护协同创新项目,48SKY00号。 |
附件 |
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| ANALYSIS OF EST-SSRs INFORMATION IN SINIPERCA CHUATSI TRANSCRIPTOME AND DETECTION OF POLYMORPHISM |
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YUAN Wen-Cheng1, HUANG He-Zhong1,2, LI Wen-Long1, LU Yao1, ZHANG Mao-You3, WANG Rong-Quan4
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1.School of Medicine and Life Sciences, Medical College of Soochow University, Fisheries Research Institute of Soochow University, Suzhou 215123, China;2.Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaian 223300, China;3.Aquaculture Technology Guide Station of Suzhou City, Jiangsu Province, Suzhou 215000, China;4.Chang-Yang Ecological Technology Development Limited Company of Suzhou City, Jiangsu Province, Suzhou 215200, China
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| Abstract: |
| To protect the germplasm resources of fish Siniperca chuatsi, we studied the transcriptome functional SSR molecular makers in high-throughput sequencing technology and detected the polymorphism of EST-SSR molecular makers. Result shows that 14094 EST-SSRs distributed in 35285 unigenes were detected, which is 27.51% of all 51245 unigenes. The total length of S. chuatsi transcriptome EST-SSRs was 195086bp, the length distribution of transcriptome EST-SSRs was from 10—25bp and the average length was 13bp. There were 90 types of repeat motifs in the transcriptome EST-SSRs, in which dinucleotide and trinucleotide repeats were two main types for taking 30.62% and 14.23% of all the SSR repeat motifs, respectively. The most abundant di-and tri-motifs were GT/AC and AAG/CCT, occupying 32.12% and 6.36% of all the SSR repeat motifs, respectively. Of 100 EST-SSR microsatellite primers of economic characters that randomly selected, 24.7% were polymorphism primers that could be developed as functional molecular makers of SSR. |
| Key words: Siniperca chuatsi transcriptome EST-SSR information polymorphism |
