| 摘要: |
| 采用ISSR 分子标记技术, 对洞庭青鲫(Carassius auratus var. Dongtingking)、野生二倍体和三倍体鲫(C. auratus)、彭泽鲫(C. auratus var. Pengze)4 个鲫品系群体共120 个个体的遗传多样性进行分析。9 个ISSR 引物在四个鲫品系群体中共获得1637 个扩增位点, 其中多态位点1572 个, 多态位点比例为96.03%。4 个鲫品系群体内的多态位点比例分别为46.14%、65.40%、73.76%和51.94%, 遗传距离分别为0.0905、0.1186、0.1351 和0.1056。在4 个鲫品系群体之间, 洞庭青鲫与彭泽鲫的遗传距离最小(0.1191), 二倍体鲫和三倍体鲫的遗传距离次之(0.1336), 而洞庭青鲫、彭泽鲫与二倍体鲫的遗传距离(分别为0.1377、0.1367)略小于它们与三倍体鲫的遗传距离(均为0.1378)。UPGMA 聚类结果为洞庭青鲫与彭泽鲫聚成一个分支, 二倍体鲫与三倍体鲫聚成另一个分支。结果表明, 洞庭青鲫、彭泽鲫等养殖群体内的遗传多样性明显低于野生鲫群体; 四个鲫品系中, 洞庭青鲫与彭泽鲫的亲缘关系较近, 二倍体鲫与三倍体鲫的亲缘关系较近, 推测洞庭青鲫、彭泽鲫、三倍体鲫均起源于二倍体鲫。二倍体洞庭青鲫和二倍体野生鲫资源的保护必须引起重视。 |
| 关键词: 洞庭青鲫 鲫品系 遗传多样性 ISSR |
| DOI：10.11693/hyhz20141000287 |
| 分类号: |
| 基金项目:国家自然科学基金项目,31372519号;湖南省高校创新平台基金项目,13K103号;环洞庭湖水产健康养殖与加工湖南省重点实验室项目,2013—2014;湖南省高校科技创新团队支持计划,2011—2014。 |
附件 |
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| GENETIC DIVERSITY OF DONGTINGKING CRUCIAN CARP AND OTHER THREE STRAINS OF CRUCIAN CARP REVEALED BY ISSR ANALYSIS |
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LIU Liang-Guo, YANG Pin-Hong, XIE Chun-Hua, WANG Xiao-Yan, XU Li-Ming
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Collaborative Innovation Center of the Aquatic Efficient Health Production in Hunan province, Key Laboratory of Zoology in Hunan Higher Education, College of Life Science, Hunan University of Arts and Science, Changde 415000, China
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| Abstract: |
| To realize the genetic diversity of different strains of Carassius auratus, inter-simple sequence repeat (ISSR) molecular marker was applied from the Dongtingking crucian carps, the diploid and triploid wild crucian carps, and Pengze crucian carps. Nine ISSR primers were used to analyze the four strains. A total of 1637 reproducible DNA fragments were amplified from all the 120 individuals, in which 1572 fragments were polymorphic, and the percentage of polymorphic loci was 96.03%. The percentages of polymorphic loci of the four strains were 46.14%, 65.40%, 73.76%, and 51.94%, and the genetic distances were 0.0905, 0.1186, 0.1351, and 0.1056, respectively. The shortest genetic distance (0.1191) occurred between Dongtingking crucian carp and Pengze crucian carp; and the longest ones were between the diploid and triploid crucian carp (0.1336), and between the Dongtingking crucian carp or Pengze crucian carp, and the diploid crucian carp (0.1377 and 0.1367, respectively) were slightly smaller than that between the Dongtingking crucian carp or Pengze crucian carp and the triploid crucian carp (both were 0.1378). The unweighted pair-group methods with arithmetic means (UPGMA) cluster analysis shows that Dongtingking crucian carp and Pengze crucian carp population gathers one group, and the diploid and triploid crucian carp population assembled to another. The results indicate that the genetic diversity of intrapopulation of the Dongtingking crucian or Pengze crucian carp was obviously lower than that of wild crucian carps. Among the four strains, the Dongtingking crucian carp was closely related with Pengze crucian carp, and so did the diploid and the triploid ones. It is speculated that the Dongtingking crucian carp, Pengze crucian carp, and triploid crucian carp were originated from a diploid wild crucian carp. Therefore, it is necessary to enhance conservation of the diploid Dongtingking crucian carp and wild crucian carp. |
| Key words: Carassius auratus var. Dongtingking strains of crucian carp genetic diversity ISSR |
