| 引用本文: | 张沛,迟美丽,温海深,钱焜,倪蒙,张亚晨,黄政举,宋志飞,柴森浩.盐度调控对花鲈(Lateolabrax maculatus)生长相关基因表达的影响.海洋与湖沼,2015,46(2):446-453. |
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| 盐度调控对花鲈(Lateolabrax maculatus)生长相关基因表达的影响 |
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张沛, 迟美丽, 温海深, 钱焜, 倪蒙, 张亚晨, 黄政举, 宋志飞, 柴森浩
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中国海洋大学水产学院 青岛 266003
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| 摘要: |
| 生长激素受体(GHR)作为GH/IGF 轴的中心环节, 在内分泌调控中发挥重要作用。本实验采用cDNA 末端快速扩增法(RACE)技术克隆出花鲈GHR1 和GHR2 的cDNA 全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24、48、96、144、192h 后, 花鲈肝脏中GHRs、IGF-1 及垂体GH 表达情况。结果表明, GHR1 cDNA 全长序列2436bp, 编码637个氨基酸; GHR2 cDNA 全长序列2940bp, 编码582 个氨基酸。GHR1 与GHR2 由信号肽、胞外区、跨膜区、胞内区组成, 且结构存在差异。脑、肾、鳃中GHR1 表达明显高于GHR2; 而在肌肉、垂体、肝脏、盲肠、胸腺、心脏中, GHR2 表达明显高于GHR1。24h 时, 各组GHR1 表达不变, GHR2、GH、IGF-1 显著下降。之后, 相对于海水组, 淡水组和半海水组GHRs 和IGF-1 表达升高, 而GH 下降, GH与GHR 负相关。据结果推测, 花鲈GHR2 可能为SL 受体, GH/IGF 轴参与低渗调控可能是通过增加GHRs, 进而激活下游IGF-1 表达而实现。 |
| 关键词: 花鲈 盐度 生长激素受体 生长激素 胰岛素样生长因子1 |
| DOI：10.11693/hyhz20140400102 |
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| 基金项目:国家“十二五”国家科技支撑计划重大项目课题,2011BAD13B03号。 |
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| CLONING OF GROWTH HORMONE RECEPTOR AND SALINITY EFFECTS ON THE EXPRESSION OF ITS RELATED GENES IN SEABASS LATEOLABRAX MACULATUS |
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ZHANG Pei, CHI Mei-Li, WEN Hai-Shen, QIAN Kun, NI Meng, ZHANG Ya-Chen, HUANG Zheng-Ju, SONG Zhi-Fei, CHAI Sen-Hao
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College of Fisheries, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| Growth hormone receptors (GHRs) as the key part of GH/IGF axis play important roles in individual endocrine. From the cDNA sequence of seabass Lateolabrax maculatus, two GHRs were cloned by rapid amplifycation of cDNA ends (RACE). Acute salinity regulation experiment contained three groups: seawater, half-seawater, and freshwater. Hepatic GHRs, insulin-like growth factor (IGF-1) and hypophyseal growth hormone (GH) were determined after 24h, 48h, 96h, 144h, 192h by qPCR. GHR1 and GHR2 were 2436bp and 2940bp in length, encoding 637 and 582 amino acid, respectively. GHR1 and GHR2 contained different putative signal peptide, extracellular domain, transmembrane domain, and intracellular domain. Expression level of GHR1 was higher than GHR2’s in brain, kidney and gill, but in muscle, pituitary, liver, caecum, thymus, and heart, the opposite. GHR1 mRNA was constant, while the levels of GHR2, GH, and IGF-1 decreased in 24h. Compared with seawater group, the half-seawater and freshwater groups had higher expression of GHRs, IGF-1 and lower expression of GH. Therefore, the GHR1 of seabass is probably the somatolactin receptor. GH/IGF axis participates in osmoregulation, and may improve the levels of GHRs, and further activate the expression of IGF-1. |
| Key words: Lateolabrax maculatus salinity growth hormone receptor growth hormone insulin-like growth factor 1 |
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