| 摘要: |
| 传染性皮下和造血器官坏死病毒(infectious hypodermal and hematopoietic necrosis virus, IHHNV)是对虾养殖的重要病原之一,可感染多种虾种。罗氏沼虾(Macrobrachium rosenbergii)是中国养殖的重要沼虾品种之一。根据国际兽疫局(Office International des Epizooties, OIE)推荐的IHHNV检测方法,在中国大陆地区首次开展IHHNV在罗氏沼虾中的感染和流行情况调查。结果显示,在研究区的罗氏沼虾养殖区, IHHNV广泛流行,阳性率高达90%;但所有成年罗氏沼虾均未表现出明显的病症,仅表现为病毒的携带。通过基因序列分析显示,检测到的华南地区毒株属于Ⅰ型感染株,与菲律宾株进化关系较为接近;华东地区毒株属于Ⅱ型感染株,与东南亚株进化关系较近。本研究为IHHNV在罗氏沼虾内的感染、流行和防控提供了详细参考。 |
| 关键词: 传染性皮下和造血器官坏死病毒(IHHNV) 罗氏沼虾 感染与流行 |
| DOI：10.11693/hyhz20150600168 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863计划)项目,2012AA020101号;浙江省重大科技专项计划项目,2013C03045-1号;宁波市自然科学基金项目,2014A610182号。 |
附件 |
|
| EPIDEMIOLOGY OF INFECTIOUS HYPODERMAL AND HEMATOPOIETIC NECROSIS VIRUS IN MACROBRACHIUM ROSENBERGII |
|
FAN Dong-Dong, WEI Yong-Wei, MIAO Liang, CHEN Jiong
|
|
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China
|
| Abstract: |
| Infectious hypodermal and haematopoietic necrosis virus(IHHNV) is one of major viral pathogens of penaeid shrimps. Giant river prawn Macrobrachium rosenbergii is one of the most important shrimp species in China. However, study and report on IHHNV infection and the epidemiology remain blank in the mainland of China. According to the method recommended by OIE(Office International des Epizooties), infection and epidemiology of IHHNV in M. rosenbergii were conducted in the mainland of China. Results show a high positive percentage of IHHNV in M. rosenbergii(90%) in the aquiculture, but no samples showed any syndrome of IHHNV infection. Sequence analysis indicated that the collected strains in South China belonged to the lineage I infectious IHHNV group, and had a close evolutionary relationship with the Philippines strains, while the collected strains in East China belonged to the lineage Ⅱ infectious IHHNV group, and had a close evolutionary relationship with the Southeast Asia strains. This study provides an insightful reference to the prevention and control of IHHNV infection in M. rosenbergii. |
| Key words: infectious hypodermal and haematopoietic necrosis virus(IHHNV) Macrobrachium rosenbergii infection and epidemiology |
