引用本文:	韦一凡,郇丽,牛建峰,何林文,黄爱优,张宝玉,林阿朋,王广策.细胞穿膜肽转导大型海藻及对其光合作用的影响.海洋与湖沼,2015,46(5):1235-1240.

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细胞穿膜肽转导大型海藻及对其光合作用的影响
韦一凡^1,2, 郇丽¹, 牛建峰¹, 何林文¹, 黄爱优¹, 张宝玉¹, 林阿朋¹, 王广策¹
1.实验海洋生物学重点实验室中国科学院海洋研究所青岛 266071;2.中国科学院大学北京 100049

摘要:

细胞穿膜肽能够介导外源物质进入细胞,是一种简单高效的分子运输手段。传统的大型海藻外源物质转导方法较为复杂,并且效率偏低。目前尚没有将细胞穿膜肽应用于大型海藻的报道。本实验利用细胞穿膜肽九聚精氨酸(R9)对浒苔、紫菜和海带这三种常见的大型海藻进行转入,观察其转导效果,并测定转导后藻体光合作用效率的变化。实验结果表明,细胞穿膜肽R9能够成功的转入三种大型海藻,转入的效率与藻体细胞壁结构有一定联系;转导后藻体的F_v/F_m、ETR(I)和ETR(II)没有明显变化,说明R9对大型海藻的光合作用没有负面影响。本实验建立了一种新型的大型海藻外源物质转导方法,为今后的大型海藻育种,分子转化以及外源物质转入机制等研究奠定了基础。

关键词: 细胞穿膜肽九聚精氨酸大型海藻光合作用效率

DOI：10.11693/hyhz20141200365

分类号:

基金项目:国家863项目,2012AA100811-5号;中国科学院科技战略先导性项目,XDA05030401号;国家海洋局公益性项目,201105008-2号,201105023-7号。

附件

TRANSLOCATION OF CELL PENETRATING PEPTIDES ON MARINE MACROALGAE AND THE IMPACT ON PHOTOSYNTHESIS OF MACROALGAE

WEI Yi-Fan^1,2, HUAN Li¹, NIU Jian-Feng¹, HE Lin-Wen¹, HUANG Ai-You¹, ZHANG Bao-Yu¹, LIN A-Peng¹, WANG Guang-Ce¹

1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China

Abstract:

Cell penetrating peptides(CPPs) are effective molecular tools for transporting exogenous substances into different cells. Traditional methods of importing exogenous substances into macroalgae are complicated and inefficient. Yet, no study has been taken to apply CPPs on macroalgae. In this study, by using cell-penetrating peptide nona-arginine(R9), we conducted a translocation experiment on three common marine macroalgae Ulva prolifera, Porphyra yezoensis, and Laminaria japonica to determine photosynthetic efficiency of R9-treated thalli on Dual-PAM fluorometer. We found that R9 could pass cell barriers of three macroalgae, and the efficiency of transductions was closely related to the structures of cell walls. It had no significant change in F_v/F_m, ETR(Ⅰ), and ETR(Ⅱ) of macroalgae after being treated with R9, demonstrating that R9 had no negative impact on the photosynthesis of macroalgae. This is a new method of importing exogenous substances into macroalgae, and may help in macroalgae breeding, molecular transformation, and exogenous substance intake.

Key words: Cell penetrating peptides(CPPs) nona-arginine marine macroalgae photosynthetic efficiency