引用本文:	曹奕,吴洪喜,陈爱华,吴杨平,张雨,王超,张志伟,姚国兴,蔡永祥.我国沿海大竹蛏(Solen grandis)野生群体及增殖放流群体遗传多样性的ISSR 分析.海洋与湖沼,2015,46(6):1398-1404.

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*我国沿海大竹蛏(Solen grandis)野生群体及增殖放流群体遗传多样性的ISSR 分析*
曹奕¹, 吴洪喜², 陈爱华¹, 吴杨平¹, 张雨¹, 王超¹, 张志伟¹, 姚国兴¹, 蔡永祥¹
1.江苏省海洋水产研究所江苏省海洋经济贝类研究开发中心南通 226007;2.浙江省海洋水产养殖研究所浙江省近岸水域生物资源开发与保护重点实验室温州 325005

摘要:

近年来, 大竹蛏野生资源因过度捕捞等原因急剧减少, 主要通过人工增殖放流方式进行补充。对我国沿海大竹蛏群体进行遗传多样性研究, 评判增殖放流活动对野生群体遗传结构的影响, 能够了解大竹蛏当前的种质资源状况, 为合理保护该资源提供科学依据。采用ISSR 分子标记技术, 对广西北海(BH)、江苏启东(QD)、山东日照(RZ)、辽宁营口(YK)、河北秦皇岛(QH)5 个野生大竹蛏群体及江苏蒋家沙增殖放流群体(JJ)共150 个样品进行了研究。实验结果表明, 我国沿海大竹蛏遗传多样性水平高, 但各野生群体间已出现显著的遗传分化; 江苏蒋家沙增殖放流群体较启东野生群体遗传多样性有所下降, 但两群体间的遗传分化并不明显。所以在苗种培育过程中, 应当保证亲本数量及品质, 扩大亲本选择区域来提高遗传多样性水平, 同时应加大本地原种保护力度来避免种质混杂。

关键词: 大竹蛏 ISSR 遗传多样性遗传结构

DOI：10.11693/hyhz20150100037

分类号:

基金项目:江苏省自然科学基金青年项目, BK2012235 号; 江苏省农业科技自主创新资金项目, CX(14)2083 号; 江苏省“六大人才高峰”项目, 2013-NY-013 号; 南通市农业科技创新项目, HL2014018 号; 浙江省近岸水域生物资源开发与保护重点实验室开放课题, J2013008 号。

附件

ISSR ANALYSIS ON GENETIC DIVERSITY OF STOCK AND WILD SOLEN GRANDIS POPULATIONS ALONG COASTAL AREAS OF CHINA

CAO Yi¹, WU Hong-Xi², CHEN Ai-Hua¹, WU Yang-Ping¹, ZHANG Yu¹, WANG Chao¹, ZHANG Zhi-Wei¹, YAO Guo-Xing¹, CAI Yong-Xiang¹

1.Jiangsu Marine Economic Shellfish Research and Development Center, Jiangsu Marine Fisheries Research Institute, Nantong 226007, China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Zhejiang Mariculture Research Institute, Wenzhou 325005, China

Abstract:

Due to overfishing and other reasons, wild Solen grandis resources decreased sharply, to which stock enhancement reproduction and release becomes a major supplemental way. To understand S. grandis germplasm resources and provide a scientific basis for the protection of S. grandis resource, we studied the genetic diversity of its populations along coastal areas of China. Five wild populations including Beihai(BH)(Guangxi), Qidong(QD)(Jiangsu), Rizhao (RZ)(Shandong), Yingkou(YK)(Liaoning), and Qinhuangdao(QH)(Hebei), and a stock population in Jiangjia sandbar (JJ)(Jiangsu), were selected and sampled for ISSR(inter-simple sequence repeat)analysis. Results show that S. grandis showed a high level of genetic diversity, and genetic differentiation among wild populations had been significant. Comparing with wild population QD, the genetic diversity of population JJ decreased, but the genetic differentiation between the two populations was not significant. Therefore, we could guarantee the quantity and quality of the parents, expand parental area to increase genetic diversity, and pay great attention to protecting the local precious germplasm not to be confounded.

Key words: Solen grandis ISSR genetic diversity genetic structure