| 摘要: |
| 染色质免疫共沉淀技术(ChIP)作为研究DNA 结合蛋白与DNA 相互作用最有力的工具, 越 来越受到重视。但目前该技术在牡蛎等贝类中的应用尚未见相关报道。为了研究长牡蛎早期胚胎发 育过程中表观遗传信息的变化模式, 本文在长牡蛎胚胎中探索并建立了一套完整的染色质免疫共沉 淀方法, 构建了高质量的高通量测序文库, 首次获得了牡蛎全基因组水平的组蛋白H3K4me3 修饰分 布, 从而为研究牡蛎的表观遗传调控奠定了基础。 |
| 关键词: 长牡蛎(Crassostrea gigas) ChIP-seq 高通量测序 组蛋白修饰 |
| DOI：10.11693/hyhz20150500141 |
| 分类号: |
| 基金项目:上海市浦江人才计划项目, 14PJ1408400 号; 同济大学985 启动项目, 2000141505 号。 |
附件 |
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| DEVELOPMENT OF A ChIP-seq METHOD IN CRASSOSTREA GIGAS |
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ZHAN Wang, WANG Wen, SHI Wei-Yang
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School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
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| Abstract: |
| Chromatin immunoprecipitation coupled with high throughput sequencing (ChIP-seq) is a powerful tool to analyze DNA-protein interactions. However, the application of ChIP-seq in Crassostrea gigas has not been investigated. In this study, we established a protocol for performing ChIP-seq experiment in oyster early embryos. We successfully performed H3K4me3 ChIP-seq on 16-cell stage oyster embryo and revealed the genome-wide distribution of the H3K4me3 histone marks. This study is the first step toward understanding the epigenetic regulation of oyster embryogenesis. |
| Key words: Crassostrea gigas ChIP-seq high-throughput sequencing histone modification |
